BBa_K2114014 1 BBa_K2114014 aGFPnano_HA_G4S_CotB 2016-10-08T11:00:00Z 2016-10-09T07:10:32Z The cotB gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 19, 2389???2401 (2010). N-terminal fusion of anti-GFP nanobody to spore crust gene cotB by a flexible GGGGS linker. false false _2582_ 32299 32299 9 false Introduction of HA epitope tag and GGGGS linker by primer extensions and PCR. false Wladislaw Stroukov igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z