BBa_K2114017 1 BBa_K2114017 aGFPnano_HA_aHelix_cgeA 2016-10-09T11:00:00Z 2016-10-18T05:04:22Z The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala et al. in 2010. [Ref] N-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by an alpha helical linker. false false _2582_ 32299 32299 9 false Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR. false Wladislaw Stroukov annotation2519009 1 cgeA range2519009 1 415 816 annotation2519006 1 anti-GFP nanobody range2519006 1 1 354 annotation2519007 1 HA range2519007 1 355 381 annotation2519008 1 alpha helical linker range2519008 1 382 414 BBa_K2114017_sequence 1 atgacaagtcaagtccaactggtggagtctggtggcgctttggtgcagccaggtggctctctgcgtttgtcctgtgccgcttctggcttcccagtgaaccgctattccatgcgctggtatcgccaggctccaggcaaagagcgtgagtgggtagccggtatgtccagcgcgggtgatcgtagctcctatgaagactccgtgaagggccgtttcaccatcagccgtgacgatgcccgtaacacggtgtatctgcaaatgaacagcttgaaacctgaagatacggccgtgtattactgtaatgtgaacgtgggcttcgagtattggggccaaggcacccaggtcaccgtctccagctacccatacgatgttccagattacgctggcggcggcgaagcggcggcgaaaggcggcggcatgagctctgaaaatgcacagttaaaaaaggatttaattaaagccgttttaagccctttatttccaactgcaacagaaggaggagaaaatatggatagtaatcttaaagccttgcttgatgctgccatcgatcaaaaagtagatgaaagtgaaacggttacggcagaatctattttagacccttctcttccggcaagatggatttttgccagaattacgccaggcactacaatcagcattgtgactgattcaggtgacatgatcggaccggttgttttcgttgctttcgatcaggttcacgggatcgtatttgtaacacaggaaagctccgtcactccggcaggccaagctacaacattaattgatgtagacaaagtagaaagcgttacgttcttttcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z