BBa_K2114020 1 BBa_K2114020 CgeA_G4S_HA_GST 2016-10-09T11:00:00Z 2016-10-18T08:59:53Z The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare). C-terminal fusion of glutathione S-transferase to spore crust gene cgeA by a flexible GGGGS linker. false false _2582_ 32299 32299 9 false Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR. false Wladislaw Stroukov annotation2520135 1 GST range2520135 1 442 1143 annotation2520133 1 GGGGS range2520133 1 400 414 annotation2520132 1 cgeA range2520132 1 1 399 annotation2520134 1 HA range2520134 1 415 441 BBa_K2114020_sequence 1 atgagctctgaaaatgcacagttaaaaaaggatttaattaaagccgttttaagccctttatttccaactgcaacagaaggaggagaaaatatggatagtaatcttaaagccttgcttgatgctgccatcgatcaaaaagtagatgaaagtgaaacggttacggcagaatctattttagacccttctcttccggcaagatggatttttgccagaattacgccaggcactacaatcagcattgtgactgattcaggtgacatgatcggaccggttgttttcgttgctttcgatcaggttcacgggatcgtatttgtaacacaggaaagctccgtcactccggcaggccaagctacaacattaattgatgtagacaaagtagaaagcgttacgttcttttcaggtggtggtggttcttacccatacgatgttccagattacgctatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggaagttctgttccaggggcccctgggatccccggaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z