BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K2115000
1
BBa_K2115000
It detects carbon monoxide
2016-10-13T11:00:00Z
2016-10-14T01:22:39Z
LacI: genomically it comes from E.coli bacterium, however we took this promoter from the IGEM catalog.
RBS: ribosome binding site.
CooA: a transcription factor present in the Rhodospirillum Rubrum protobacteria.
Terminators: an RNA sequence that usually occurs at the end of a gene or operon mRNA and causes transcription to stop.
This composite is made by LacI promoter, which is inducible by IPTG molecule; After LacI promoter is activated,it will also activate the transcription of CooA, a transcription factor that can detect carbon monoxide.
LacI promoter is an indispensble part, since without it the bacteria would be in an unnecessary operation.
false
false
_2583_
33161
33161
9
false
The only consideration was the bioinformatic assembly this parts, because we had to learn to use Artemis software.
false
Antonella Elizabeth Osses Toledo; Javier David Villacreses Lucero
component2509072
1
BBa_R0010
component2509088
1
BBa_B0010
component2509083
1
BBa_K352001
component2509084
1
BBa_B0012
component2509080
1
BBa_B0030
annotation2509084
1
BBa_B0012
range2509084
1
907
947
annotation2509083
1
BBa_K352001
range2509083
1
230
898
annotation2509088
1
BBa_B0010
range2509088
1
956
1035
annotation2509080
1
BBa_B0030
range2509080
1
209
223
annotation2509072
1
BBa_R0010
range2509072
1
1
200
BBa_K352001
1
BBa_K352001
CooA from Rhodospirillum rubrum
2010-10-01T11:00:00Z
2015-05-08T01:12:11Z
Hwan Youn, Robert L. Kerby, Mary Conrad, et al.
2004. Functionally Critical Elements of CooA-Related
CO Sensors. J. Bacteriol. 186(5):1320-1329.
doi:10.1128/JB.186.5.1320-1329.2004.
CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow
on CO as a sole energy source.
CooA is a member of a family of transcriptional regulators similar to the cAMP receptor protein
and fumavate nitrate reduction from Escherichia coli. The protein is active in sequence-specific DNA binding in the presence of CO, but not in the absence of CO. The protein to be a dimer in the absence of CO. The product, CooA, is 28% identical (51% similar) to CRP(cAMP receptor protein) and 18% identical (45% similar) to FNR(fumavate nitrate reduction) from Escherichia coli.
Inactive Fe(II) CooA structure adapted from that of the strain with PDB identification no. 1FT9. The protein consists of two monomers, shaded differently, which dimerize along the central C-helices of adjacent effector-binding domains. The solved structure is asymmetric, in which one monomer contains fused C- and D-helices. Nonetheless, both F-helices that interact with DNA in a sequence-specific manner are buried from the surface in the structure. The 4/5 loop is noted and so are the Pro2 and His77 heme Fe(II) ligands.
false
false
_474_606_
0
5815
9
It's complicated
true
The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
false
Cihan Tastan
annotation2081251
1
Start Codon
range2081251
1
1
3
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K2115000_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgccaccacgcttcaatattgcaaacgtactgctgtctcctgatggtgagacgttcttccgcggttttcgctctaagattcatgcgaaaggctctctggtatgtactggtgaaggtgatgaaaacggtgtttttgttgtggttgatggtcgcctgcgtgtttacctggttggtgaggagcgtgagattagcctgttctacctgacttccggcgatatgttctgcatgcattccggctgcctggttgaagccaccgagcgcaccgaagtgcgtttcgccgatatccgcacgttcgagcagaaactgcaaacctgtccgtctatggcatggggcctgatcgccattctgggccgtgctctgacctcctgtatgcgtaccatcgaagacctgatgttccacgatattaaacaacgtatcgcgggctttttcatcgaccacgctaacactaccggtcgccagactcagggtggcgtaattgtttctgttgacttcactgtagaggaaatcgctaatctgatcggtagctcccgccagactactagcacggcgctgaactctctgattaaagagggttacatctcccgccagggccgtggtcactatactatcccgaacctggttcgcctgaaggcggctgcggatggtgaccgcgatgacgatgacgattgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K352001_sequence
1
atgccaccacgcttcaatattgcaaacgtactgctgtctcctgatggtgagacgttcttccgcggttttcgctctaagattcatgcgaaaggctctctggtatgtactggtgaaggtgatgaaaacggtgtttttgttgtggttgatggtcgcctgcgtgtttacctggttggtgaggagcgtgagattagcctgttctacctgacttccggcgatatgttctgcatgcattccggctgcctggttgaagccaccgagcgcaccgaagtgcgtttcgccgatatccgcacgttcgagcagaaactgcaaacctgtccgtctatggcatggggcctgatcgccattctgggccgtgctctgacctcctgtatgcgtaccatcgaagacctgatgttccacgatattaaacaacgtatcgcgggctttttcatcgaccacgctaacactaccggtcgccagactcagggtggcgtaattgtttctgttgacttcactgtagaggaaatcgctaatctgatcggtagctcccgccagactactagcacggcgctgaactctctgattaaagagggttacatctcccgccagggccgtggtcactatactatcccgaacctggttcgcctgaaggcggctgcggatggtgaccgcgatgacgatgacgattga
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z