BBa_K2115004
type: ComponentDefinition
persistent identity: BBa K2115004
display id: BBa_K2115004
version: 1
was generated by: iGEM to SBOL conversion
description: high levels of Carbon monoxide detection
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T02:39:43Z
mutable provenance: pCooM:it comes from the CooA transcription factor from Rhospirillum rubrum RBS: ribosome binding site Pink chromoprotein: LuxR: a part of the quorum sensing system Terminator: an RNA sequence that usually occ
owned by: james
owned by: myers
mutable description: pCooM promoter is regulated by CooA when there are high levels of carbon monoxide in the environment, when this promoter is activated a pink chromoprotein and a LuxR promoter will be transcribed. The expression of LuxR is going to bind to the AHL molecule that allows the transcription of the plux promoter (BBa_K2115002)
discontinued: false
dominant: false
group u list: _2583_
m user id: 33161
owner id: 33161
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Adding LuxR to later activate pLux and then activating the third level of carbon monoxide detection.
star: false
creator: Antonella Elizabeth Osses Toledo
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
component: BBa_K592012
component: BBa_K352003
sequence annotation: BBa_K352003
sequence annotation: BBa_B0034
sequence annotation: BBa_K592012
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K2115004_sequence/1

BBa_B0012
type: Component
persistent identity: component2509363
display id: component2509363
version: 1
top level: BBa_K2115004
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2509361
display id: component2509361
version: 1
top level: BBa_K2115004
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component2509357
display id: component2509357
version: 1
top level: BBa_K2115004
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2509354
display id: component2509354
version: 1
top level: BBa_K2115004
definition: BBa_B0034
access: public

BBa_K592012
type: Component
persistent identity: component2509352
display id: component2509352
version: 1
top level: BBa_K2115004
definition: eforRed
access: public

BBa_B0034
type: Component
persistent identity: component2509350
display id: component2509350
version: 1
top level: BBa_K2115004
definition: BBa_B0034
access: public

BBa_K352003
type: Component
persistent identity: component2509348
display id: component2509348
version: 1
top level: BBa_K2115004
definition: BBa_K352003
access: public

BBa_K352003
type: SequenceAnnotation
persistent identity: annotation2509348
display id: annotation2509348
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509348/range2509348/1
component: BBa_K352003

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509348/range2509348/1
type: Range
persistent identity: range2509348
display id: range2509348
version: 1
top level: BBa_K2115004
direction: direction/reverse
start: 1
end: 66
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2509350
display id: annotation2509350
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509350/range2509350/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509350/range2509350/1
type: Range
persistent identity: range2509350
display id: range2509350
version: 1
top level: BBa_K2115004
direction: direction/reverse
start: 75
end: 86
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2509352
display id: annotation2509352
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509352/range2509352/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509352/range2509352/1
type: Range
persistent identity: range2509352
display id: range2509352
version: 1
top level: BBa_K2115004
direction: direction/reverse
start: 93
end: 773
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2509354
display id: annotation2509354
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509354/range2509354/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509354/range2509354/1
type: Range
persistent identity: range2509354
display id: range2509354
version: 1
top level: BBa_K2115004
direction: direction/reverse
start: 782
end: 793
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2509357
display id: annotation2509357
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509357/range2509357/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509357/range2509357/1
type: Range
persistent identity: range2509357
display id: range2509357
version: 1
top level: BBa_K2115004
direction: direction/forward
start: 800
end: 1555
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2509361
display id: annotation2509361
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509361/range2509361/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509361/range2509361/1
type: Range
persistent identity: range2509361
display id: range2509361
version: 1
top level: BBa_K2115004
direction: direction/forward
start: 1589
end: 1668
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2509363
display id: annotation2509363
version: 1
top level: BBa_K2115004
location: https://synbiohub.org/public/igem/BBa_K2115004/annotation2509363/range2509363/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2115004/annotation2509363/range2509363/1
type: Range
persistent identity: range2509363
display id: range2509363
version: 1
top level: BBa_K2115004
direction: direction/forward
start: 1677
end: 1717
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_K352003
type: ComponentDefinition
persistent identity: BBa K352003
display id: BBa_K352003
version: 1
was generated by: iGEM to SBOL conversion
description: pCooM Promoter from Rhodospirillum rubrum
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
mutable provenance: Y. He, T. Gaal, R. Karls, T. Donohue, R. Gourse and G.P. Roberts, J. Biol. Chem. 274 (1999), pp. 10840???10846.
owned by: james
owned by: myers
mutable description: The two CooA-regulated R. rubrum promoters, PcooF and PcooM, contain 2-fold symmetric DNA sequences that serve as CooA-binding sites and are similar to the CRP consensus sequence. This is consistent with the similarity between CooA and CRP in their helix-turn-helix motifs. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo. The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
discontinued: false
dominant: false
experience: experience/None
group u list: _474_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
star: false
creator: Ceren Seref
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CooA binding Site
sequence: https://synbiohub.org/public/igem/BBa_K352003_sequence/1

CooA binding Site
type: SequenceAnnotation
persistent identity: annotation2081259
display id: annotation2081259
version: 1
top level: BBa_K352003
location: https://synbiohub.org/public/igem/BBa_K352003/annotation2081259/range2081259/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K352003/annotation2081259/range2081259/1
type: Range
persistent identity: range2081259
display id: range2081259
version: 1
top level: BBa_K352003
direction: direction/forward
start: 14
end: 18
orientation: inline

CooA binding Site
type: SequenceAnnotation
persistent identity: annotation2081260
display id: annotation2081260
version: 1
top level: BBa_K352003
location: https://synbiohub.org/public/igem/BBa_K352003/annotation2081260/range2081260/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K352003/annotation2081260/range2081260/1
type: Range
persistent identity: range2081260
display id: range2081260
version: 1
top level: BBa_K352003
direction: direction/forward
start: 25
end: 29
orientation: inline

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: iGEM to SBOL conversion
description: eforRed, red chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: coming soon
owned by: james
owned by: myers
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: coming soon
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
top level: eforRed
direction: direction/forward
start: 1
end: 681
orientation: inline

https://synbiohub.org/public/igem/BBa_K2115004_sequence/1
type: Sequence
persistent identity: BBa K2115004 sequence
display id: BBa_K2115004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acagcatgacgagtgtcggccaagtgacggaattttccgcccaggtggcgtactcccgttctcacttactagagaaagaggagaaatactagatgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctcccatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K352003_sequence/1
type: Sequence
persistent identity: BBa K352003 sequence
display id: BBa_K352003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acagcatgacgagtgtcggccaagtgacggaattttccgcccaggtggcgtactcccgttctcact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592012_sequence/1
type: Sequence
persistent identity: BBa K592012 sequence
display id: BBa_K592012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z