##gff-version 3
##sequence-region BBa_K2115005 1 1601
BBa_K2115005	.	ribosome_entry_site	103	117	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2115005	.	ribosome_entry_site	103	117	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2115005	.	engineered_region	103	117	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2115005	.	ribosome_entry_site	110	114	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2115005	.	CDS	124	792	.	+	0	ID=BBa_K592009;Name=BBa_K592009
BBa_K2115005	.	CDS	124	789	.	+	0	ID=annotation2131628;Name=amilCP;Parent=BBa_K592009
BBa_K2115005	.	CDS	822	1439	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K2115005	.	engineered_region	822	1439	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K2115005	.	tag	1401	1432	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K2115005	.	CDS	822	1400	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K2115005	.	engineered_tag	1440	1464	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K2115005	.	terminator	1522	1601	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K2115005	.	stem_loop	1533	1576	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2115005	.	engineered_region	1522	1601	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2115005	.	ribosome_entry_site	801	815	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2115005	.	ribosome_entry_site	801	815	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2115005	.	engineered_region	801	815	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2115005	.	ribosome_entry_site	808	812	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2115005	.	terminator	1473	1513	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K2115005	.	stop_codon	1506	1506	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2115005	.	polyA_site	1500	1513	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2115005	.	stem_loop	1480	1499	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2115005	.	engineered_region	1473	1513	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2115005	.	promoter	1	94	.	+	0	ID=BBa_K352002;Name=BBa_K352002
BBa_K2115005	.	non_covalent_binding_site	41	44	.	+	0	ID=annotation2081253;Name=CooA binding site;Parent=BBa_K352002
BBa_K2115005	.	conserved_region	23	91	.	+	0	ID=annotation2081258;Name=Protected by CooA+RNAP;Parent=BBa_K352002
BBa_K2115005	.	non_covalent_binding_site	52	56	.	+	0	ID=annotation2081254;Name=CooA binding site;Parent=BBa_K352002
BBa_K2115005	.	sequence_feature	79	84	.	+	0	ID=annotation2081255;Name=-10 Region;Parent=BBa_K352002
BBa_K2115005	.	conserved_region	36	61	.	+	0	ID=annotation2081257;Name=Protected by CooA;Parent=BBa_K352002
BBa_K2115005	.	start_codon	92	93	.	+	0	ID=annotation2081256;Name=Transcription Start;Parent=BBa_K352002
BBa_K2115005	.	sequence_feature	8	33	.	+	0	ID=annotation2081252;Name=A+T rich sequence;Parent=BBa_K352002
>BBa_K2115005
ttcggcgtcttttcatacccccataaaaactctggataactgtcatctggccgacagacggggccgggctttttgtcgc
ttactcggcgccaggtactagagattaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggt
ttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcag
acggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaa
gcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagag
gatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccat
gtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactg
agcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactattt
gtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggat
gtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaat
aatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggag
gagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacc
ttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctg
gcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaa
gatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatta
caatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagc
aatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatact
aaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacg
ctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctc