LOCUS       BBa_K2116024            1888 bp    DNA     linear       22-Nov-2024
DEFINITION  Dual fluorescence reporter for bxb1 integrase
ACCESSION   BBa_K2116024
VERSION     BBa_K2116024.1  
FEATURES             Location/Qualifiers
     terminator      1..105
                     /label=BBa_K1897030
     CDS             106..852
                     /label=mNectarine
     misc_feature    106..111
                     /label=TAATAA stop codons
     misc_feature    850..852
                     /label=ATG start codon
     CDS             106..852
                     /label=BBa_K2116997
     RBS             853..884
                     /label=BBa_K2116020
     promoter        885..1022
                     /label=BBa_K2116021
     RBS             1023..1057
                     /label=BBa_K2116019
     misc_feature    1058..1060
                     /label=ATG start codon
     misc_feature    1778..1783
                     /label=TAATAA stop codon
     CDS             1058..1783
                     /label=sfGFP
     CDS             1058..1783
                     /label=BBa_K2116017
     terminator      1784..1888
                     /label=BBa_K1897030
ORIGIN
        1 agtagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
       61 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaataataa ttcttcgcag
      121 tcgcatcaaa agcagcaatc gtcggaatat ctcgagcagg tagggtgggc acgacactgc
      181 cggaaggcgc gcaagcatgt cgagttgcta acacatcagg agcaataccc tttcttatag
      241 ctcaaattac agctgcaata tacgtgggcc attgacgtgt ccaaaaaaac gaaacattca
      301 acaaaactga aggcgcagca ttactggcgg caggaactcg aaattcgcaa cgtactaaag
      361 cggaaactca cgcggcagga ggcctacgta tgcaagccat gaccggaggg ttggttgcca
      421 cgctgtgacg tattgacctg gtagtgaccc ttttaaacat ggtgcctcaa actggaacat
      481 ttacttgagc ggcagaacgt ctctccttag gacgcaatgc cattgttggg gcggcaggag
      541 tttcaagtag tgtgcaaggg tcaaatttgg aagtcccttg ctgtcaaagt ctatcaggcc
      601 ttatagccgg cctacaaagt gcatgcgaaa cgaaggtatc gtcttaactc cactgtccta
      661 tagggtgcgc ttgccttcac caggtgggaa gcactgaaag tcaaaccgac aaacacatgg
      721 gagcatgcct gccgggaggg ggagcggaag ttagagcttg agtacgggca actgccttgg
      781 gaggtatact tgaaactttg cgtatttgag gaactattac cggtacaata gaagaagcgg
      841 aaacgattgg tagccgagag gcgcttacat agggaattac tcaatcggcc ggcttgtcga
      901 cgacggcggt ctccgtcgtc aggatcatcc gggcgctagc acaataccta ggactgagct
      961 agccgtcaag ggtttgtacc gtacaccact gagaccgcgg tggttgacca gacaaaccac
     1021 gatctaaata aaagcgtata aataagagga ggttatcatg agcaaaggag aagaactttt
     1081 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     1141 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg
     1201 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt
     1261 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat
     1321 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac
     1381 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat
     1441 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca
     1501 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg
     1561 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat
     1621 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc
     1681 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg
     1741 gattacacat ggcatggatg agctctacaa aaggccttaa taaagtagtc ttggactcct
     1801 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     1861 ccgccgggcg ttttttattg gtgagaat
//