LOCUS BBa_K2116024 1888 bp DNA linear 05-Nov-2024 DEFINITION Dual fluorescence reporter for bxb1 integrase ACCESSION BBa_K2116024 VERSION BBa_K2116024.1 FEATURES Location/Qualifiers terminator 1..105 /label=BBa_K1897030 CDS 106..852 /label=mNectarine misc_feature 106..111 /label=TAATAA stop codons misc_feature 850..852 /label=ATG start codon CDS 106..852 /label=BBa_K2116997 RBS 853..884 /label=BBa_K2116020 promoter 885..1022 /label=BBa_K2116021 RBS 1023..1057 /label=BBa_K2116019 misc_feature 1058..1060 /label=ATG start codon misc_feature 1778..1783 /label=TAATAA stop codon CDS 1058..1783 /label=sfGFP CDS 1058..1783 /label=BBa_K2116017 terminator 1784..1888 /label=BBa_K1897030 ORIGIN 1 agtagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg 61 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaataataa ttcttcgcag 121 tcgcatcaaa agcagcaatc gtcggaatat ctcgagcagg tagggtgggc acgacactgc 181 cggaaggcgc gcaagcatgt cgagttgcta acacatcagg agcaataccc tttcttatag 241 ctcaaattac agctgcaata tacgtgggcc attgacgtgt ccaaaaaaac gaaacattca 301 acaaaactga aggcgcagca ttactggcgg caggaactcg aaattcgcaa cgtactaaag 361 cggaaactca cgcggcagga ggcctacgta tgcaagccat gaccggaggg ttggttgcca 421 cgctgtgacg tattgacctg gtagtgaccc ttttaaacat ggtgcctcaa actggaacat 481 ttacttgagc ggcagaacgt ctctccttag gacgcaatgc cattgttggg gcggcaggag 541 tttcaagtag tgtgcaaggg tcaaatttgg aagtcccttg ctgtcaaagt ctatcaggcc 601 ttatagccgg cctacaaagt gcatgcgaaa cgaaggtatc gtcttaactc cactgtccta 661 tagggtgcgc ttgccttcac caggtgggaa gcactgaaag tcaaaccgac aaacacatgg 721 gagcatgcct gccgggaggg ggagcggaag ttagagcttg agtacgggca actgccttgg 781 gaggtatact tgaaactttg cgtatttgag gaactattac cggtacaata gaagaagcgg 841 aaacgattgg tagccgagag gcgcttacat agggaattac tcaatcggcc ggcttgtcga 901 cgacggcggt ctccgtcgtc aggatcatcc gggcgctagc acaataccta ggactgagct 961 agccgtcaag ggtttgtacc gtacaccact gagaccgcgg tggttgacca gacaaaccac 1021 gatctaaata aaagcgtata aataagagga ggttatcatg agcaaaggag aagaactttt 1081 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 1141 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg 1201 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt 1261 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat 1321 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac 1381 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat 1441 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca 1501 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg 1561 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat 1621 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc 1681 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg 1741 gattacacat ggcatggatg agctctacaa aaggccttaa taaagtagtc ttggactcct 1801 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg 1861 ccgccgggcg ttttttattg gtgagaat //