LOCUS       BBa_K2116028            1941 bp    DNA     linear       22-Nov-2024
DEFINITION  bxb1 integrase under the control of nitric oxide and AHL 
            responsive AND gate
ACCESSION   BBa_K2116028
VERSION     BBa_K2116028.1  
FEATURES             Location/Qualifiers
     misc_feature    130..147
                     /label=esabox
     misc_feature    148..157
                     /label=NorR binding site 3 (low affinity)
     misc_feature    161..177
                     /label=NorR binding site 2 (low affinity)
     misc_feature    188..208
                     /label=NorR binding site 1 (high affinity)
     misc_feature    249..281
                     /label=sigma 54 binding site
     misc_feature    301..318
                     /label=esabox
     promoter        1..318
                     /label=AND gate
     promoter        1..318
                     /label=BBa_K2116011
     RBS             319..330
                     /label=BBa_J61100
     misc_feature    331..333
                     /label=ATG start codon
     CDS             331..1836
                     /label=Bxb1
     misc_feature    1831..1836
                     /label=TAATAA stop codons
     CDS             331..1836
                     /label=BBa_K2116026
     terminator      1837..1941
                     /label=BBa_K1897030
ORIGIN
        1 cgtagcaacg cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga
       61 aaacgatcct ggtgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca
      121 acggaaaaac ctgtactata gtgcaggtca atttgactat agatattgtc atatcgacca
      181 tttgattgat agtcattttg actactcatt aatgggcata attttattta tagagtaaaa
      241 acaatcagat aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagggtacc
      301 cctgtactat agtgcaggaa agaggggaca atgcgtgcac tggttgttat tcgtctgagc
      361 cgtgttaccg atgcaaccac cagtccggaa cgtcagctgg aaagctgtca gcagctgtgt
      421 gcacagcgtg gttgggatgt tgttggtgtt gcagaggatc tggatgttag cggtgcagtt
      481 gatccgtttg atcgtaaacg tcgtccgaat ctggcacgtt ggctggcatt tgaagaacag
      541 ccgtttgatg ttattgttgc ctatcgtgtt gatcgtctga cccgtagcat tcgtcatctg
      601 caacagctgg ttcattgggc agaagatcat aaaaaactgg ttgtgagcgc aaccgaagca
      661 cattttgata ccaccacccc gtttgcagca gttgttattg cactgatggg caccgttgca
      721 cagatggaac tggaagcaat taaagaacgt aatcgtagcg cagcccattt taacattcgt
      781 gcaggtaaat atcgtggtag cctgcctccg tggggttatc tgccgacgcg tgtagatggt
      841 gaatggcgtc tggttcctga cccggttcag cgtgaacgta ttctggaagt atatcatcgt
      901 gtggtggata atcatgaacc gctgcatctg gttgcacatg atctgaatcg tcgtggtgtt
      961 ctgagtccca aagattattt tgctcagctg caaggtcgtg aaccgcaggg tcgtgaatgg
     1021 tctgcaaccg cactgaaacg tagcatgatt agcgaagcaa tgctgggtta tgcaaccctg
     1081 aatggtaaaa ccgttcgtga tgatgatggt gcaccgctgg ttcgtgcaga accgattctg
     1141 acacgtgaac agctggaagc actgcgtgcc gaactggtta aaaccagccg tgcaaaaccg
     1201 gcagttagca ccccgagcct gctgctgcgt gttctgtttt gtgcagtttg tggtgaaccg
     1261 gcatacaaat ttgccggtgg tggtcgtaaa catccgcgtt atcgttgtcg tagcatgggt
     1321 tttccgaaac attgtggtaa tggtacagtt gcaatggcag aatgggatgc attttgcgaa
     1381 gaacaggttc tggatctgct gggtgatgcc gaacgtctgg aaaaagtttg ggttgcaggt
     1441 agcgatagcg cagttgaact ggccgaagtt aatgcggaac tggtcgatct caccagtctg
     1501 attggaagtc ccgcatatcg tgcgggtagt cctcagcgtg aagcactgga tgcacgtatt
     1561 gcagcactgg cagcacgtca agaagaactg gaaggtctgg aagcacgtcc gagcggttgg
     1621 gaatggcgtg aaacaggtca gcgttttggt gattggtggc gtgagcagga taccgcagca
     1681 aaaaatacct ggctgcgtag tatgaatgtt cgcctgacct ttgatgttcg cggtggcctg
     1741 acccgcacca ttgattttgg cgatctgcaa gaatatgaac agcatctgcg tctgggtagc
     1801 gttgttgaac gtctgcatac cggcatgagc taataaagta gtcttggact cctgttgata
     1861 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1921 gcgtttttta ttggtgagaa t
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