BBa_K2116028 1 BBa_K2116028 bxb1 integrase under the control of nitric oxide and AHL responsive AND gate 2016-10-13T11:00:00Z 2016-10-28T07:58:53Z E.coli, bxb1?, esabox AND gate, bxb1 with moderately fast degradation tag. Useful for constructing logic gates. esabox can be replaced to create other AND gates with NO responsiveness [link to our lactate AND gate]. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu component2530663 1 BBa_J61100 component2530667 1 BBa_K2116026 component2530662 1 BBa_K2116011 component2530668 1 BBa_K1897030 annotation2530662 1 BBa_K2116011 range2530662 1 1 318 annotation2530668 1 BBa_K1897030 range2530668 1 1837 1941 annotation2530667 1 BBa_K2116026 range2530667 1 331 1836 annotation2530663 1 BBa_J61100 range2530663 1 319 330 BBa_K2116011 1 BBa_K2116011 AND gate regulated by Nitric Oxide and 3OC6HSL 2016-10-13T11:00:00Z 2016-10-24T07:38:58Z PnorV from E.coli, esaboxes from []. rAND gate created by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox is added to create competitive binding between norR and esaR, and improve AND gate behaviour. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2512423 1 NorR binding site 3 (low affinity) range2512423 1 148 157 annotation2528974 1 AND gate range2528974 1 1 318 annotation2512426 1 sigma 54 binding site range2512426 1 249 281 annotation2512424 1 NorR binding site 2 (low affinity) range2512424 1 161 177 annotation2512422 1 esabox range2512422 1 130 147 annotation2512427 1 esabox range2512427 1 301 318 annotation2512425 1 NorR binding site 1 (high affinity) range2512425 1 188 208 BBa_J61100 1 BBa_J61100 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A {{JCA_Arkin_RBSFamily}} false true _95_ 0 483 95 In stock false N/A true John Anderson BBa_K1897030 1 BBa_K1897030 Lambda t0 terminator 2016-10-12T11:00:00Z 2016-10-13T07:53:33Z Source Lambda t0 terminator false false _2363_ 29424 29424 9 false Considerations false Keshiniy Madivannan BBa_K2116026 1 BBa_K2116026 bxb1 integrase 2016-10-13T11:00:00Z 2016-10-24T04:58:09Z lambda phage bxb1 integrate that can invert a sequence flanked by the att sites it recognises. false false _2584_ 31332 31336 9 false degradation tags? false Asli Azizoglu annotation2528904 1 TAATAA stop codons range2528904 1 1501 1506 annotation2528902 1 ATG start codon range2528902 1 1 3 annotation2528903 1 Bxb1 range2528903 1 1 1506 BBa_K2116028_sequence 1 cgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaacctgtactatagtgcaggtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggaaagaggggacaatgcgtgcactggttgttattcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcgtggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccgaatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgtagcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattttgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaagaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgccgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgtggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattattttgctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaatgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattctgacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagcctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgcgttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttgcgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagttgaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctcagcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgagcggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctggctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaagaatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagctaataaagtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_K2116026_sequence 1 atgcgtgcactggttgttattcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcgtggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccgaatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgtagcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattttgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaagaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgccgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgtggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattattttgctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaatgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattctgacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagcctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgcgttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttgcgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagttgaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctcagcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgagcggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctggctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaagaatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagctaataa BBa_J61100_sequence 1 aaagaggggaca BBa_K1897030_sequence 1 agtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_K2116011_sequence 1 cgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaacctgtactatagtgcaggtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z