LOCUS       BBa_K2116041            1086 bp    DNA     linear       22-Dec-2024
DEFINITION  AND gate 108
ACCESSION   BBa_K2116041
VERSION     BBa_K2116041.1  
FEATURES             Location/Qualifiers
     misc_feature    130..147
                     /label=esabox
     misc_feature    148..157
                     /label=NorR binding site 3 (low affinity)
     misc_feature    161..177
                     /label=NorR binding site 2 (low affinity)
     misc_feature    188..208
                     /label=NorR binding site 1 (high affinity)
     misc_feature    249..281
                     /label=sigma 54 binding site
     misc_feature    301..318
                     /label=esabox
     promoter        1..318
                     /label=AND gate
     promoter        1..318
                     /label=BBa_K2116011
     misc_feature    327..354
                     /label=BBa_K2116090
     misc_feature    361..363
                     /label=ATG start codon
     misc_feature    1081..1086
                     /label=TAATAA stop codon
     CDS             361..1086
                     /label=sfGFP
     CDS             361..1086
                     /label=BBa_K2116017
ORIGIN
        1 cgtagcaacg cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga
       61 aaacgatcct ggtgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca
      121 acggaaaaac ctgtactata gtgcaggtca atttgactat agatattgtc atatcgacca
      181 tttgattgat agtcattttg actactcatt aatgggcata attttattta tagagtaaaa
      241 acaatcagat aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagggtacc
      301 cctgtactat agtgcaggta ctagagaaaa gcgtataaat aagaggaggt tatctactag
      361 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      421 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
      481 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
      541 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
      601 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      661 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      721 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
      781 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
      841 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
      901 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      961 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     1021 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct
     1081 taataa
//