BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520523 1 sfGFP range2520523 1 1 726 annotation2520521 1 ATG start codon range2520521 1 1 3 annotation2520522 1 TAATAA stop codon range2520522 1 721 726 BBa_K2116041 1 BBa_K2116041 AND gate 108 2016-10-18T11:00:00Z 2016-10-26T05:28:09Z synthesized PnorV_esabox_spacer false false _2584_ 31332 31332 9 false none false Lukas Schmidheini component2530375 1 BBa_K2116090 component2530374 1 BBa_K2116011 component2530379 1 BBa_K2116017 annotation2530374 1 BBa_K2116011 range2530374 1 1 318 annotation2530379 1 BBa_K2116017 range2530379 1 361 1086 annotation2530375 1 BBa_K2116090 range2530375 1 327 354 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116011 1 BBa_K2116011 AND gate regulated by Nitric Oxide and 3OC6HSL 2016-10-13T11:00:00Z 2016-10-24T07:38:58Z PnorV from E.coli, esaboxes from []. rAND gate created by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox is added to create competitive binding between norR and esaR, and improve AND gate behaviour. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2512423 1 NorR binding site 3 (low affinity) range2512423 1 148 157 annotation2528974 1 AND gate range2528974 1 1 318 annotation2512427 1 esabox range2512427 1 301 318 annotation2512422 1 esabox range2512422 1 130 147 annotation2512424 1 NorR binding site 2 (low affinity) range2512424 1 161 177 annotation2512425 1 NorR binding site 1 (high affinity) range2512425 1 188 208 annotation2512426 1 sigma 54 binding site range2512426 1 249 281 BBa_K2116041_sequence 1 cgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaacctgtactatagtgcaggtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggtactagagaaaagcgtataaataagaggaggttatctactagatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116011_sequence 1 cgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaacctgtactatagtgcaggtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcagg BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z