LOCUS       BBa_K2116056            1572 bp    DNA     linear       22-Dec-2024
DEFINITION  Bxb1 with ssrA tag under Tet promoter
ACCESSION   BBa_K2116056
VERSION     BBa_K2116056.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    55..66
                     /label=BBa_K2116092
     misc_feature    67..69
                     /label=ATG start codon
     CDS             67..1572
                     /label=Bxb1
     misc_feature    1567..1572
                     /label=TAATAA stop codons
     CDS             67..1572
                     /label=BBa_K2116026
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacaaagag
       61 gggacaatgc gtgcactggt tgttattcgt ctgagccgtg ttaccgatgc aaccaccagt
      121 ccggaacgtc agctggaaag ctgtcagcag ctgtgtgcac agcgtggttg ggatgttgtt
      181 ggtgttgcag aggatctgga tgttagcggt gcagttgatc cgtttgatcg taaacgtcgt
      241 ccgaatctgg cacgttggct ggcatttgaa gaacagccgt ttgatgttat tgttgcctat
      301 cgtgttgatc gtctgacccg tagcattcgt catctgcaac agctggttca ttgggcagaa
      361 gatcataaaa aactggttgt gagcgcaacc gaagcacatt ttgataccac caccccgttt
      421 gcagcagttg ttattgcact gatgggcacc gttgcacaga tggaactgga agcaattaaa
      481 gaacgtaatc gtagcgcagc ccattttaac attcgtgcag gtaaatatcg tggtagcctg
      541 cctccgtggg gttatctgcc gacgcgtgta gatggtgaat ggcgtctggt tcctgacccg
      601 gttcagcgtg aacgtattct ggaagtatat catcgtgtgg tggataatca tgaaccgctg
      661 catctggttg cacatgatct gaatcgtcgt ggtgttctga gtcccaaaga ttattttgct
      721 cagctgcaag gtcgtgaacc gcagggtcgt gaatggtctg caaccgcact gaaacgtagc
      781 atgattagcg aagcaatgct gggttatgca accctgaatg gtaaaaccgt tcgtgatgat
      841 gatggtgcac cgctggttcg tgcagaaccg attctgacac gtgaacagct ggaagcactg
      901 cgtgccgaac tggttaaaac cagccgtgca aaaccggcag ttagcacccc gagcctgctg
      961 ctgcgtgttc tgttttgtgc agtttgtggt gaaccggcat acaaatttgc cggtggtggt
     1021 cgtaaacatc cgcgttatcg ttgtcgtagc atgggttttc cgaaacattg tggtaatggt
     1081 acagttgcaa tggcagaatg ggatgcattt tgcgaagaac aggttctgga tctgctgggt
     1141 gatgccgaac gtctggaaaa agtttgggtt gcaggtagcg atagcgcagt tgaactggcc
     1201 gaagttaatg cggaactggt cgatctcacc agtctgattg gaagtcccgc atatcgtgcg
     1261 ggtagtcctc agcgtgaagc actggatgca cgtattgcag cactggcagc acgtcaagaa
     1321 gaactggaag gtctggaagc acgtccgagc ggttgggaat ggcgtgaaac aggtcagcgt
     1381 tttggtgatt ggtggcgtga gcaggatacc gcagcaaaaa atacctggct gcgtagtatg
     1441 aatgttcgcc tgacctttga tgttcgcggt ggcctgaccc gcaccattga ttttggcgat
     1501 ctgcaagaat atgaacagca tctgcgtctg ggtagcgttg ttgaacgtct gcataccggc
     1561 atgagctaat aa
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