LOCUS       BBa_K2116059            1864 bp    DNA     linear       17-Dec-2024
DEFINITION  and gate_bxb1(medium tag)
ACCESSION   BBa_K2116059
VERSION     BBa_K2116059.1  
FEATURES             Location/Qualifiers
     misc_feature    149..158
                     /label=NorR binding 3 low affinity
     misc_feature    162..178
                     /label=NorR binding 2 low affinity
     misc_feature    189..209
                     /label=NorR binding 1 high affinity
     misc_feature    302..319
                     /label=esabox
     misc_feature    250..282
                     /label=sigma54 binding site
     promoter        1..285
                     /label=PnorV
     promoter        1..319
                     /label=BBa_K2116004
     misc_feature    326..328
                     /label=ATG start codon
     CDS             326..1864
                     /label=bxb1
     misc_feature    1859..1864
                     /label=TAATAA stop codons
     misc_feature    1838..1864
                     /label=ssrA tag (moderat)
     CDS             326..1864
                     /label=BBa_K2116030
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagggtac
      301 ccctgtacta tagtgcaggt actagatgcg tgcactggtt gttattcgtc tgagccgtgt
      361 taccgatgca accaccagtc cggaacgtca gctggaaagc tgtcagcagc tgtgtgcaca
      421 gcgtggttgg gatgttgttg gtgttgcaga ggatctggat gttagcggtg cagttgatcc
      481 gtttgatcgt aaacgtcgtc cgaatctggc acgttggctg gcatttgaag aacagccgtt
      541 tgatgttatt gttgcctatc gtgttgatcg tctgacccgt agcattcgtc atctgcaaca
      601 gctggttcat tgggcagaag atcataaaaa actggttgtg agcgcaaccg aagcacattt
      661 tgataccacc accccgtttg cagcagttgt tattgcactg atgggcaccg ttgcacagat
      721 ggaactggaa gcaattaaag aacgtaatcg tagcgcagcc cattttaaca ttcgtgcagg
      781 taaatatcgt ggtagcctgc ctccgtgggg ttatctgccg acgcgtgtag atggtgaatg
      841 gcgtctggtt cctgacccgg ttcagcgtga acgtattctg gaagtatatc atcgtgtggt
      901 ggataatcat gaaccgctgc atctggttgc acatgatctg aatcgtcgtg gtgttctgag
      961 tcccaaagat tattttgctc agctgcaagg tcgtgaaccg cagggtcgtg aatggtctgc
     1021 aaccgcactg aaacgtagca tgattagcga agcaatgctg ggttatgcaa ccctgaatgg
     1081 taaaaccgtt cgtgatgatg atggtgcacc gctggttcgt gcagaaccga ttctgacacg
     1141 tgaacagctg gaagcactgc gtgccgaact ggttaaaacc agccgtgcaa aaccggcagt
     1201 tagcaccccg agcctgctgc tgcgtgttct gttttgtgca gtttgtggtg aaccggcata
     1261 caaatttgcc ggtggtggtc gtaaacatcc gcgttatcgt tgtcgtagca tgggttttcc
     1321 gaaacattgt ggtaatggta cagttgcaat ggcagaatgg gatgcatttt gcgaagaaca
     1381 ggttctggat ctgctgggtg atgccgaacg tctggaaaaa gtttgggttg caggtagcga
     1441 tagcgcagtt gaactggccg aagttaatgc ggaactggtc gatctcacca gtctgattgg
     1501 aagtcccgca tatcgtgcgg gtagtcctca gcgtgaagca ctggatgcac gtattgcagc
     1561 actggcagca cgtcaagaag aactggaagg tctggaagca cgtccgagcg gttgggaatg
     1621 gcgtgaaaca ggtcagcgtt ttggtgattg gtggcgtgag caggataccg cagcaaaaaa
     1681 tacctggctg cgtagtatga atgttcgcct gacctttgat gttcgcggtg gcctgacccg
     1741 caccattgat tttggcgatc tgcaagaata tgaacagcat ctgcgtctgg gtagcgttgt
     1801 tgaacgtctg cataccggca tgagcgctgc taacgacgaa aactacgctg acgcttctta
     1861 ataa
//