BBa_K2116030 1 BBa_K2116030 bxb1 integrase with moderately fast degradation tag 2016-10-13T11:00:00Z 2016-10-19T01:14:07Z ... degradation tag to avoid leakiness. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520563 1 ssrA tag (moderat) range2520563 1 1513 1539 annotation2520551 1 TAATAA stop codons range2520551 1 1534 1539 annotation2520550 1 bxb1 range2520550 1 1 1539 annotation2520549 1 ATG start codon range2520549 1 1 3 BBa_K2116059 1 BBa_K2116059 and gate_bxb1(medium tag) 2016-10-18T11:00:00Z 2016-10-19T08:33:49Z - - false false _2584_ 31332 31332 9 false - false Lukas Schmidheini component2523531 1 BBa_K2116004 component2523536 1 BBa_K2116030 annotation2523531 1 BBa_K2116004 range2523531 1 1 319 annotation2523536 1 BBa_K2116030 range2523536 1 326 1864 BBa_K2116004 1 BBa_K2116004 AND gate regulated by norR and esaR (one esabox) 2016-10-12T11:00:00Z 2016-10-24T11:43:03Z Different variations of the AND gate can be found here []. This AND gate was constructed using PnorV [enter link] and one esabox, positioned after the transcription start site to serve as a roadblock. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520167 1 sigma54 binding site range2520167 1 250 282 annotation2529026 1 PnorV range2529026 1 1 285 annotation2520163 1 NorR binding 3 low affinity range2520163 1 149 158 annotation2520165 1 NorR binding 1 high affinity range2520165 1 189 209 annotation2520164 1 NorR binding 2 low affinity range2520164 1 162 178 annotation2520166 1 esabox range2520166 1 302 319 BBa_K2116030_sequence 1 atgcgtgcactggttgttattcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcgtggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccgaatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgtagcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattttgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaagaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgccgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgtggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattattttgctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaatgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattctgacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagcctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgcgttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttgcgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagttgaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctcagcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgagcggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctggctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaagaatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagcgctgctaacgacgaaaactacgctgacgcttcttaataa BBa_K2116059_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggtactagatgcgtgcactggttgttattcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcgtggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccgaatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgtagcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattttgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaagaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgccgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgtggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattattttgctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaatgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattctgacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagcctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgcgttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttgcgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagttgaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctcagcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgagcggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctggctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaagaatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagcgctgctaacgacgaaaactacgctgacgcttcttaataa BBa_K2116004_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggggtacctctaaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z