LOCUS       BBa_K2116060            1858 bp    DNA     linear       22-Nov-2024
DEFINITION  AND gate bxb1 (fast tag)
ACCESSION   BBa_K2116060
VERSION     BBa_K2116060.1  
FEATURES             Location/Qualifiers
     misc_feature    149..158
                     /label=NorR binding 3 low affinity
     misc_feature    162..178
                     /label=NorR binding 2 low affinity
     misc_feature    189..209
                     /label=NorR binding 1 high affinity
     misc_feature    302..319
                     /label=esabox
     misc_feature    250..282
                     /label=sigma54 binding site
     promoter        1..285
                     /label=PnorV
     promoter        1..319
                     /label=BBa_K2116004
     CDS             320..1858
                     /label=Bxb1
     misc_feature    320..322
                     /label=ATG start codon
     misc_feature    1853..1858
                     /label=TAATAA stop codons
     misc_feature    1832..1858
                     /label=ssrA tag (very fast)
     CDS             320..1858
                     /label=BBa_K2116009
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagggtac
      301 ccctgtacta tagtgcagga tgcgtgcact ggttgttatt cgtctgagcc gtgttaccga
      361 tgcaaccacc agtccggaac gtcagctgga aagctgtcag cagctgtgtg cacagcgtgg
      421 ttgggatgtt gttggtgttg cagaggatct ggatgttagc ggtgcagttg atccgtttga
      481 tcgtaaacgt cgtccgaatc tggcacgttg gctggcattt gaagaacagc cgtttgatgt
      541 tattgttgcc tatcgtgttg atcgtctgac ccgtagcatt cgtcatctgc aacagctggt
      601 tcattgggca gaagatcata aaaaactggt tgtgagcgca accgaagcac attttgatac
      661 caccaccccg tttgcagcag ttgttattgc actgatgggc accgttgcac agatggaact
      721 ggaagcaatt aaagaacgta atcgtagcgc agcccatttt aacattcgtg caggtaaata
      781 tcgtggtagc ctgcctccgt ggggttatct gccgacgcgt gtagatggtg aatggcgtct
      841 ggttcctgac ccggttcagc gtgaacgtat tctggaagta tatcatcgtg tggtggataa
      901 tcatgaaccg ctgcatctgg ttgcacatga tctgaatcgt cgtggtgttc tgagtcccaa
      961 agattatttt gctcagctgc aaggtcgtga accgcagggt cgtgaatggt ctgcaaccgc
     1021 actgaaacgt agcatgatta gcgaagcaat gctgggttat gcaaccctga atggtaaaac
     1081 cgttcgtgat gatgatggtg caccgctggt tcgtgcagaa ccgattctga cacgtgaaca
     1141 gctggaagca ctgcgtgccg aactggttaa aaccagccgt gcaaaaccgg cagttagcac
     1201 cccgagcctg ctgctgcgtg ttctgttttg tgcagtttgt ggtgaaccgg catacaaatt
     1261 tgccggtggt ggtcgtaaac atccgcgtta tcgttgtcgt agcatgggtt ttccgaaaca
     1321 ttgtggtaat ggtacagttg caatggcaga atgggatgca ttttgcgaag aacaggttct
     1381 ggatctgctg ggtgatgccg aacgtctgga aaaagtttgg gttgcaggta gcgatagcgc
     1441 agttgaactg gccgaagtta atgcggaact ggtcgatctc accagtctga ttggaagtcc
     1501 cgcatatcgt gcgggtagtc ctcagcgtga agcactggat gcacgtattg cagcactggc
     1561 agcacgtcaa gaagaactgg aaggtctgga agcacgtccg agcggttggg aatggcgtga
     1621 aacaggtcag cgttttggtg attggtggcg tgagcaggat accgcagcaa aaaatacctg
     1681 gctgcgtagt atgaatgttc gcctgacctt tgatgttcgc ggtggcctga cccgcaccat
     1741 tgattttggc gatctgcaag aatatgaaca gcatctgcgt ctgggtagcg ttgttgaacg
     1801 tctgcatacc ggcatgagcg ctgctaacga cgaaaactac gctctggctg cttaataa
//