BBa_K2116060 1 BBa_K2116060 AND gate bxb1 (fast tag) 2016-10-18T11:00:00Z 2016-10-19T08:33:57Z - - false false _2584_ 31332 31332 9 false - false Lukas Schmidheini component2523624 1 BBa_K2116004 component2523629 1 BBa_K2116009 annotation2523624 1 BBa_K2116004 range2523624 1 1 319 annotation2523629 1 BBa_K2116009 range2523629 1 320 1858 BBa_K2116004 1 BBa_K2116004 AND gate regulated by norR and esaR (one esabox) 2016-10-12T11:00:00Z 2016-10-24T11:43:03Z Different variations of the AND gate can be found here []. This AND gate was constructed using PnorV [enter link] and one esabox, positioned after the transcription start site to serve as a roadblock. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520167 1 sigma54 binding site range2520167 1 250 282 annotation2520166 1 esabox range2520166 1 302 319 annotation2520163 1 NorR binding 3 low affinity range2520163 1 149 158 annotation2520164 1 NorR binding 2 low affinity range2520164 1 162 178 annotation2520165 1 NorR binding 1 high affinity range2520165 1 189 209 annotation2529026 1 PnorV range2529026 1 1 285 BBa_K2116009 1 BBa_K2116009 bxb1 integrase, with very fast degradation tag 2016-10-12T11:00:00Z 2016-10-19T01:55:21Z bxb1 comes from []. Codon optimised and synthesised bxb1 integrase, codon optimized for E.coli. false false _2584_ 31332 31336 9 false codon optimized false Asli Azizoglu annotation2520472 1 ssrA tag (very fast) range2520472 1 1513 1539 annotation2520467 1 TAATAA stop codons range2520467 1 1534 1539 annotation2520466 1 ATG start codon range2520466 1 1 3 annotation2520465 1 Bxb1 range2520465 1 1 1539 BBa_K2116009_sequence 1 atgcgtgcactggttgttattcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcgtggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccgaatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgtagcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattttgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaagaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgccgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgtggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattattttgctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaatgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattctgacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagcctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgcgttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttgcgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagttgaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctcagcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgagcggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctggctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaagaatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagcgctgctaacgacgaaaactacgctctggctgcttaataa BBa_K2116004_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggggtacctctaaat BBa_K2116060_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggatgcgtgcactggttgttattcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcgtggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccgaatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgtagcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattttgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaagaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgccgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgtggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattattttgctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaatgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattctgacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagcctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgcgttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttgcgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagttgaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctcagcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgagcggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctggctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaagaatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagcgctgctaacgacgaaaactacgctctggctgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z