LOCUS       BBa_K2116061            1599 bp    DNA     linear       22-Dec-2024
DEFINITION  Bxb1 with moderate degradation tag under Tet promoter
ACCESSION   BBa_K2116061
VERSION     BBa_K2116061.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    61..63
                     /label=ATG start codon
     CDS             61..1599
                     /label=bxb1
     misc_feature    1594..1599
                     /label=TAATAA stop codons
     misc_feature    1573..1599
                     /label=ssrA tag (moderat)
     CDS             61..1599
                     /label=BBa_K2116030
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atgcgtgcac tggttgttat tcgtctgagc cgtgttaccg atgcaaccac cagtccggaa
      121 cgtcagctgg aaagctgtca gcagctgtgt gcacagcgtg gttgggatgt tgttggtgtt
      181 gcagaggatc tggatgttag cggtgcagtt gatccgtttg atcgtaaacg tcgtccgaat
      241 ctggcacgtt ggctggcatt tgaagaacag ccgtttgatg ttattgttgc ctatcgtgtt
      301 gatcgtctga cccgtagcat tcgtcatctg caacagctgg ttcattgggc agaagatcat
      361 aaaaaactgg ttgtgagcgc aaccgaagca cattttgata ccaccacccc gtttgcagca
      421 gttgttattg cactgatggg caccgttgca cagatggaac tggaagcaat taaagaacgt
      481 aatcgtagcg cagcccattt taacattcgt gcaggtaaat atcgtggtag cctgcctccg
      541 tggggttatc tgccgacgcg tgtagatggt gaatggcgtc tggttcctga cccggttcag
      601 cgtgaacgta ttctggaagt atatcatcgt gtggtggata atcatgaacc gctgcatctg
      661 gttgcacatg atctgaatcg tcgtggtgtt ctgagtccca aagattattt tgctcagctg
      721 caaggtcgtg aaccgcaggg tcgtgaatgg tctgcaaccg cactgaaacg tagcatgatt
      781 agcgaagcaa tgctgggtta tgcaaccctg aatggtaaaa ccgttcgtga tgatgatggt
      841 gcaccgctgg ttcgtgcaga accgattctg acacgtgaac agctggaagc actgcgtgcc
      901 gaactggtta aaaccagccg tgcaaaaccg gcagttagca ccccgagcct gctgctgcgt
      961 gttctgtttt gtgcagtttg tggtgaaccg gcatacaaat ttgccggtgg tggtcgtaaa
     1021 catccgcgtt atcgttgtcg tagcatgggt tttccgaaac attgtggtaa tggtacagtt
     1081 gcaatggcag aatgggatgc attttgcgaa gaacaggttc tggatctgct gggtgatgcc
     1141 gaacgtctgg aaaaagtttg ggttgcaggt agcgatagcg cagttgaact ggccgaagtt
     1201 aatgcggaac tggtcgatct caccagtctg attggaagtc ccgcatatcg tgcgggtagt
     1261 cctcagcgtg aagcactgga tgcacgtatt gcagcactgg cagcacgtca agaagaactg
     1321 gaaggtctgg aagcacgtcc gagcggttgg gaatggcgtg aaacaggtca gcgttttggt
     1381 gattggtggc gtgagcagga taccgcagca aaaaatacct ggctgcgtag tatgaatgtt
     1441 cgcctgacct ttgatgttcg cggtggcctg acccgcacca ttgattttgg cgatctgcaa
     1501 gaatatgaac agcatctgcg tctgggtagc gttgttgaac gtctgcatac cggcatgagc
     1561 gctgctaacg acgaaaacta cgctgacgct tcttaataa
//