##gff-version 3
##sequence-region BBa_K2116061 1 1599
BBa_K2116061	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2116061	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2116061	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2116061	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2116061	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2116061	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2116061	.	CDS	61	1599	.	+	0	ID=BBa_K2116030;Name=BBa_K2116030
BBa_K2116061	.	start_codon	61	63	.	+	0	ID=annotation2520549;Name=ATG start codon;Parent=BBa_K2116030
BBa_K2116061	.	tag	1573	1599	.	+	0	ID=annotation2520563;Name=ssrA tag (moderat);Parent=BBa_K2116030
BBa_K2116061	.	stop_codon	1594	1599	.	+	0	ID=annotation2520551;Name=TAATAA stop codons;Parent=BBa_K2116030
BBa_K2116061	.	CDS	61	1599	.	+	0	ID=annotation2520550;Name=bxb1;Parent=BBa_K2116030
>BBa_K2116061
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtgcactggttgtta
ttcgtctgagccgtgttaccgatgcaaccaccagtccggaacgtcagctggaaagctgtcagcagctgtgtgcacagcg
tggttgggatgttgttggtgttgcagaggatctggatgttagcggtgcagttgatccgtttgatcgtaaacgtcgtccg
aatctggcacgttggctggcatttgaagaacagccgtttgatgttattgttgcctatcgtgttgatcgtctgacccgta
gcattcgtcatctgcaacagctggttcattgggcagaagatcataaaaaactggttgtgagcgcaaccgaagcacattt
tgataccaccaccccgtttgcagcagttgttattgcactgatgggcaccgttgcacagatggaactggaagcaattaaa
gaacgtaatcgtagcgcagcccattttaacattcgtgcaggtaaatatcgtggtagcctgcctccgtggggttatctgc
cgacgcgtgtagatggtgaatggcgtctggttcctgacccggttcagcgtgaacgtattctggaagtatatcatcgtgt
ggtggataatcatgaaccgctgcatctggttgcacatgatctgaatcgtcgtggtgttctgagtcccaaagattatttt
gctcagctgcaaggtcgtgaaccgcagggtcgtgaatggtctgcaaccgcactgaaacgtagcatgattagcgaagcaa
tgctgggttatgcaaccctgaatggtaaaaccgttcgtgatgatgatggtgcaccgctggttcgtgcagaaccgattct
gacacgtgaacagctggaagcactgcgtgccgaactggttaaaaccagccgtgcaaaaccggcagttagcaccccgagc
ctgctgctgcgtgttctgttttgtgcagtttgtggtgaaccggcatacaaatttgccggtggtggtcgtaaacatccgc
gttatcgttgtcgtagcatgggttttccgaaacattgtggtaatggtacagttgcaatggcagaatgggatgcattttg
cgaagaacaggttctggatctgctgggtgatgccgaacgtctggaaaaagtttgggttgcaggtagcgatagcgcagtt
gaactggccgaagttaatgcggaactggtcgatctcaccagtctgattggaagtcccgcatatcgtgcgggtagtcctc
agcgtgaagcactggatgcacgtattgcagcactggcagcacgtcaagaagaactggaaggtctggaagcacgtccgag
cggttgggaatggcgtgaaacaggtcagcgttttggtgattggtggcgtgagcaggataccgcagcaaaaaatacctgg
ctgcgtagtatgaatgttcgcctgacctttgatgttcgcggtggcctgacccgcaccattgattttggcgatctgcaag
aatatgaacagcatctgcgtctgggtagcgttgttgaacgtctgcataccggcatgagcgctgctaacgacgaaaacta
cgctgacgcttcttaataa