LOCUS       BBa_K2116062            1617 bp    DNA     linear       07-Jul-2024
DEFINITION  Ptet tp901(med tag)
ACCESSION   BBa_K2116062
VERSION     BBa_K2116062.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    55..57
                     /label=ATG start codon
     misc_feature    1612..1617
                     /label=TAATA stop codons
     misc_feature    1579..1617
                     /label=ssrA tag (very fast)
     CDS             55..1617
                     /label=TP901
     CDS             55..1617
                     /label=BBa_K2116010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatgaaa
       61 catcatcacc atcatcatca ggcaggcacc aaaaaagttg caatttatac ccgtgttagc
      121 accaccaatc aggcagaaga gggttttagc attgatgagc agattgatcg tctgaccaaa
      181 tatgcagaag caatgggttg gcaggttagc gatacctata ccgatgcagg ttttagcggc
      241 gcaaaactgg aacgtcccgc aatgcagcgt ctgatcaatg atattgaaaa caaagccttc
      301 gacaccgtgc tggtgtataa actcgatcgt ctgtcacgta gcgttcgtga tacgctgtat
      361 ctggttaaag atgtgttcac taaaaacaaa atcgacttta tcagcctgaa cgagagcatt
      421 gataccagta gcgcaatggg tagcctattt ctgaccattc tgagcgcaat taatgaattt
      481 gaacgcgaga acatcaaaga acgcatgacc atgggtaaac tgggtcgtgc aaaaagcggt
      541 aaaagcatga tgtggaccaa aaccgcattt ggctattatc ataatcgcaa aaccggcatt
      601 ctggaaattg ttccgctgca agcaaccatt gttgaacaaa tctttaccga ttatctgagc
      661 ggtattagcc tgaccaaact gcgtgataaa ctgaatgaaa gcggtcatat tggcaaagat
      721 attccgtggt catatcgtac cctgcgtcag accctggata atccggttta ttgtggttat
      781 atcaaattca aagacagcct gtttgagggc atgcataaac cgatcattcc gtatgaaacc
      841 tatctgaaag ttcagaaaga actggaagaa cgtcagcagc agacctatga acgtaataac
      901 aatccgcgtc cgtttcaggc caaatatatg ctgagtggta tggcacgttg tggctattgt
      961 ggtgctccgc tgaaaattgt tctgggtcat aaacgtaaag atggtagccg taccatgaaa
     1021 tatcattgcg caaatcgttt tccgcgtaaa accaaaggta taacggtgta caacgataat
     1081 aaaaaatgtg atagcggcac ctatgacctg agcaatctgg aaaataccgt gatcgacaat
     1141 ctgatcgggt tccaagaaaa taacgacagc ctgctgaaaa tcattaatgg caataatcag
     1201 ccgattctgg atacgagcag cttcaaaaag caaatcagcc agatcgacaa aaaaatccag
     1261 aaaaacagcg atctgtacct caacgatttt atcaccatgg atgaactgaa agatcgtacc
     1321 gatagcctcc aagcagaaaa aaaactgctg aaagctaaaa tcagcgagaa caaatttaac
     1381 gatagcaccg atgtttttga actggttaag acacagctgg gtagcattcc gattaatgaa
     1441 ctgagctatg acaacaaaaa aaaaattgtg aacaacctgg tgagcaaagt tgatgttacc
     1501 gcagataatg tggacatcat ctttaaattc caactggcaa cgggtgcagc aaaagatgaa
     1561 aattatgcac tggcagccgc tgctaacgac gaaaactacg ctctggctgc ttaataa
//