LOCUS BBa_K2116063 1566 bp DNA linear 22-Nov-2024 DEFINITION Bxb1 under Tet promoter ACCESSION BBa_K2116063 VERSION BBa_K2116063.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 61..63 /label=ATG start codon CDS 61..1566 /label=Bxb1 misc_feature 1561..1566 /label=TAATAA stop codons CDS 61..1566 /label=BBa_K2116026 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atgcgtgcac tggttgttat tcgtctgagc cgtgttaccg atgcaaccac cagtccggaa 121 cgtcagctgg aaagctgtca gcagctgtgt gcacagcgtg gttgggatgt tgttggtgtt 181 gcagaggatc tggatgttag cggtgcagtt gatccgtttg atcgtaaacg tcgtccgaat 241 ctggcacgtt ggctggcatt tgaagaacag ccgtttgatg ttattgttgc ctatcgtgtt 301 gatcgtctga cccgtagcat tcgtcatctg caacagctgg ttcattgggc agaagatcat 361 aaaaaactgg ttgtgagcgc aaccgaagca cattttgata ccaccacccc gtttgcagca 421 gttgttattg cactgatggg caccgttgca cagatggaac tggaagcaat taaagaacgt 481 aatcgtagcg cagcccattt taacattcgt gcaggtaaat atcgtggtag cctgcctccg 541 tggggttatc tgccgacgcg tgtagatggt gaatggcgtc tggttcctga cccggttcag 601 cgtgaacgta ttctggaagt atatcatcgt gtggtggata atcatgaacc gctgcatctg 661 gttgcacatg atctgaatcg tcgtggtgtt ctgagtccca aagattattt tgctcagctg 721 caaggtcgtg aaccgcaggg tcgtgaatgg tctgcaaccg cactgaaacg tagcatgatt 781 agcgaagcaa tgctgggtta tgcaaccctg aatggtaaaa ccgttcgtga tgatgatggt 841 gcaccgctgg ttcgtgcaga accgattctg acacgtgaac agctggaagc actgcgtgcc 901 gaactggtta aaaccagccg tgcaaaaccg gcagttagca ccccgagcct gctgctgcgt 961 gttctgtttt gtgcagtttg tggtgaaccg gcatacaaat ttgccggtgg tggtcgtaaa 1021 catccgcgtt atcgttgtcg tagcatgggt tttccgaaac attgtggtaa tggtacagtt 1081 gcaatggcag aatgggatgc attttgcgaa gaacaggttc tggatctgct gggtgatgcc 1141 gaacgtctgg aaaaagtttg ggttgcaggt agcgatagcg cagttgaact ggccgaagtt 1201 aatgcggaac tggtcgatct caccagtctg attggaagtc ccgcatatcg tgcgggtagt 1261 cctcagcgtg aagcactgga tgcacgtatt gcagcactgg cagcacgtca agaagaactg 1321 gaaggtctgg aagcacgtcc gagcggttgg gaatggcgtg aaacaggtca gcgttttggt 1381 gattggtggc gtgagcagga taccgcagca aaaaatacct ggctgcgtag tatgaatgtt 1441 cgcctgacct ttgatgttcg cggtggcctg acccgcacca ttgattttgg cgatctgcaa 1501 gaatatgaac agcatctgcg tctgggtagc gttgttgaac gtctgcatac cggcatgagc 1561 taataa //