LOCUS       BBa_K2116065             833 bp    DNA     linear       22-Nov-2024
DEFINITION  p98
ACCESSION   BBa_K2116065
VERSION     BBa_K2116065.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             55..86
                     /label=BBa_K2116094
     misc_feature    87..89
                     /label=ATG start codon
     misc_feature    828..833
                     /label=TAATAA stop codons
     CDS             87..833
                     /label=mNectarine
     misc_feature    795..833
                     /label=ssrA tag (moderate)
     CDS             87..833
                     /label=BBa_K2116018
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgccgag
       61 aggcgcttac atagggaatt actcaaatgg ttagcaaagg cgaagaagat aacatggcca
      121 ttatcaagga gtttatgcgt ttcaaagttc atatggaggg ttccgtcaac gggcatgagt
      181 tcgagattga aggcgagggg gagggccgtc cgtacgaggg tacacaaaca gccaaactga
      241 aagtcacgaa gggtggacca cttccgttcg cgtgggatat cctgtcacct caattctgct
      301 atggaagcaa agcgtacgtg aaacatccgg ccgatattcc ggactatctg aaactgtcgt
      361 tccctgaagg tttaaactgg gaacgtgtga tgaactttga ggacggcggg gttgttaccg
      421 taacgcagga ttcctctctg caagacggcg agttcattta caaggtcaaa ctccgtggta
      481 caaattttcc cagtgatggt ccagttatgc agtgtcgcac cgttggttgg gaggccagta
      541 ccgaacgtat gcatccggag gacggcgcac tcaaaggcga aatcatgcaa cgcttaaagc
      601 tcaaggacgg cggtcattac gacgcggaag tcaaaacaac ttacaaagca aaaaaacctg
      661 tgcagttacc gggtgcatat aacgtcgaca ttaaactcga tattctttcc cataacgagg
      721 actacacaat cgttgagctg tacgaacgcg cggaaggccg tcacagcacg ggtgggatgg
      781 acgagctcta taaggctgct aacgacgaaa actacgctga cgcttcttaa taa
//