BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520523 1 sfGFP range2520523 1 1 726 annotation2520521 1 ATG start codon range2520521 1 1 3 annotation2520522 1 TAATAA stop codon range2520522 1 721 726 BBa_K2116070 1 BBa_K2116070 Pconst_esabox_sfgfp(p16comp) 2016-10-20T11:00:00Z 2016-10-24T11:16:11Z ... ... false false _2584_ 31332 31336 9 false .. false Asli Azizoglu component2529075 1 BBa_K2116017 component2529071 1 BBa_K2116090 component2529070 1 BBa_K2116025 annotation2529071 1 BBa_K2116090 range2529071 1 73 100 annotation2529075 1 BBa_K2116017 range2529075 1 101 826 annotation2529070 1 BBa_K2116025 range2529070 1 1 72 BBa_K2116025 1 BBa_K2116025 EsaR repressible promoter 2016-10-13T11:00:00Z 2016-10-16T04:39:52Z The Anderson promoter sequences were taken form the registry. Esabox sequence comes from the Pantoea stewartii ssp. stewartii. An esabox is an 18bp sequence to which the transcriptional regulator EsaR can bind. We placed one esabox right after the normally constitutive Anderson promoter [Bba_J23118] to create an EsaR repressible promoter. Transcription can be initiated by the specific AHL EsaR responds to [N-(3-oxo-hexanoyl)-L-homoserine lactone]. false false _2584_ 31336 31336 9 false The esabox has to be placed after the transcription start site to be able to act as a roadblock. false Asli Azizoglu annotation2512420 1 Anderson Promoter, BBa_J23118 range2512420 1 1 35 annotation2512421 1 esabox range2512421 1 41 58 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116070_sequence 1 ttgacggctagctcagtcctaggtattgtgctagcggtacccctgtactatagtgcaggggtacctctaaataaaagcgtataaataagaggaggttatcatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116025_sequence 1 ttgacggctagctcagtcctaggtattgtgctagcggtacccctgtactatagtgcaggggtacctctaaat BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z