BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520521 1 ATG start codon range2520521 1 1 3 annotation2520522 1 TAATAA stop codon range2520522 1 721 726 annotation2520523 1 sfGFP range2520523 1 1 726 BBa_K2116004 1 BBa_K2116004 AND gate regulated by norR and esaR (one esabox) 2016-10-12T11:00:00Z 2016-10-24T11:43:03Z Different variations of the AND gate can be found here []. This AND gate was constructed using PnorV [enter link] and one esabox, positioned after the transcription start site to serve as a roadblock. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520167 1 sigma54 binding site range2520167 1 250 282 annotation2529026 1 PnorV range2529026 1 1 285 annotation2520163 1 NorR binding 3 low affinity range2520163 1 149 158 annotation2520165 1 NorR binding 1 high affinity range2520165 1 189 209 annotation2520164 1 NorR binding 2 low affinity range2520164 1 162 178 annotation2520166 1 esabox range2520166 1 302 319 BBa_K2116074 1 BBa_K2116074 PnorV_(native)spacer_esabox_spacer_sfgfp(p21) 2016-10-20T11:00:00Z 2016-10-24T11:45:36Z p21 p21 false false _2584_ 31332 31336 9 false p21 false Asli Azizoglu component2529231 1 BBa_K2116090 component2529230 1 BBa_K2116004 component2529235 1 BBa_K2116017 annotation2529231 1 BBa_K2116090 range2529231 1 333 360 annotation2529230 1 BBa_K2116004 range2529230 1 1 332 annotation2529235 1 BBa_K2116017 range2529235 1 361 1086 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116004_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggggtacctctaaat BBa_K2116074_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggggtacctctaaataaaagcgtataaataagaggaggttatcatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z