LOCUS       BBa_K2116075            1112 bp    DNA     linear       22-Nov-2024
DEFINITION  PnorV_(native)spacer_esabox_(8bp)spacer_esabox_spacer_sfgfp(p22)
ACCESSION   BBa_K2116075
VERSION     BBa_K2116075.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=norV promoter
     misc_feature    149..158
                     /label=NorR binding 3 low affinity
     misc_feature    162..178
                     /label=NorR bindin 2 low affinity
     misc_feature    189..209
                     /label=NorR binding 1 high affinity
     misc_feature    250..282
                     /label=sigma54 binding site
     misc_feature    302..319
                     /label=esabox
     misc_feature    320..327
                     /label=spacer
     misc_feature    328..345
                     /label=esabox
     promoter        1..358
                     /label=BBa_K2116005
     misc_feature    359..386
                     /label=BBa_K2116090
     misc_feature    387..389
                     /label=ATG start codon
     misc_feature    1107..1112
                     /label=TAATAA stop codon
     CDS             387..1112
                     /label=sfGFP
     CDS             387..1112
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagggtac
      301 ccctgtacta tagtgcaggt agagtctcct gtactatagt gcaggggtac ctctaaataa
      361 aagcgtataa ataagaggag gttatcatga gcaaaggaga agaacttttc actggagttg
      421 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag
      481 agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa
      541 aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt
      601 cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
      661 atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag
      721 tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag
      781 aagatggaaa cattcttgga cacaaactcg agtacaactt taactcacac aatgtataca
      841 tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg
      901 aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      961 ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca
     1021 acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg
     1081 gcatggatga gctctacaaa aggccttaat aa
//