BBa_K2116075 1 BBa_K2116075 PnorV_(native)spacer_esabox_(8bp)spacer_esabox_spacer_sfgfp(p22) 2016-10-20T11:00:00Z 2016-10-24T11:56:08Z dgs asf false false _2584_ 31332 31336 9 false g false Asli Azizoglu component2529339 1 BBa_K2116090 component2529343 1 BBa_K2116017 component2529338 1 BBa_K2116005 annotation2529338 1 BBa_K2116005 range2529338 1 1 358 annotation2529339 1 BBa_K2116090 range2529339 1 359 386 annotation2529343 1 BBa_K2116017 range2529343 1 387 1112 BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520523 1 sfGFP range2520523 1 1 726 annotation2520521 1 ATG start codon range2520521 1 1 3 annotation2520522 1 TAATAA stop codon range2520522 1 721 726 BBa_K2116005 1 BBa_K2116005 AND gate regulated by norR and esaR (two esaboxes) 2016-10-12T11:00:00Z 2016-10-24T11:54:28Z PnorV comes from E.coli genome, esaboxes from [] AND gate constructed by placing 2 esaboxes after the transcription start site of PnorV. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520172 1 sigma54 binding site range2520172 1 250 282 annotation2520175 1 esabox range2520175 1 302 319 annotation2520171 1 NorR binding 1 high affinity range2520171 1 189 209 annotation2520170 1 NorR bindin 2 low affinity range2520170 1 162 178 annotation2520168 1 norV promoter range2520168 1 1 285 annotation2520176 1 spacer range2520176 1 320 327 annotation2520169 1 NorR binding 3 low affinity range2520169 1 149 158 annotation2520177 1 esabox range2520177 1 328 345 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116005_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggtagagtctcctgtactatagtgcaggggtacctctaaat BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc BBa_K2116075_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggtagagtctcctgtactatagtgcaggggtacctctaaataaaagcgtataaataagaggaggttatcatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z