LOCUS       BBa_K2116076            1138 bp    DNA     linear       22-Nov-2024
DEFINITION  PnorV_(native)spacer_esabox_(8bp)spacer_esabox_(8bp)spacer_esabox_R
            BS_sfgfp(23)
ACCESSION   BBa_K2116076
VERSION     BBa_K2116076.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=norV promoter
     misc_feature    149..158
                     /label=NorR binding 3 low affinity
     misc_feature    162..178
                     /label=NorR binding 2 low affinity
     misc_feature    189..209
                     /label=NorR binding 1 high affinity
     misc_feature    250..282
                     /label=sigma54
     misc_feature    302..319
                     /label=esabox
     misc_feature    320..327
                     /label=Spacer
     misc_feature    328..345
                     /label=esabox
     misc_feature    346..353
                     /label=Spacer
     misc_feature    354..371
                     /label=esabox
     promoter        1..384
                     /label=BBa_K2116006
     misc_feature    385..412
                     /label=BBa_K2116090
     misc_feature    413..415
                     /label=ATG start codon
     misc_feature    1133..1138
                     /label=TAATAA stop codon
     CDS             413..1138
                     /label=sfGFP
     CDS             413..1138
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagggtac
      301 ccctgtacta tagtgcaggt atcacgacct gtactatagt gcaggtaatc cagcctgtac
      361 tatagtgcag gggtacctct aaataaaagc gtataaataa gaggaggtta tcatgagcaa
      421 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      481 tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac
      541 ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac
      601 tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt
      661 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga
      721 cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat
      781 cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgagta
      841 caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc
      901 taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag accattatca
      961 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac
     1021 acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt
     1081 tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaaaggc cttaataa
//