BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520522 1 TAATAA stop codon range2520522 1 721 726 annotation2520521 1 ATG start codon range2520521 1 1 3 annotation2520523 1 sfGFP range2520523 1 1 726 BBa_K2116006 1 BBa_K2116006 AND gate regulated by norR and esaR (three esaboxes) 2016-10-12T11:00:00Z 2016-10-25T12:00:55Z PnorV comes from E.coli. esabox from [] AND gate constructed by placing esaboxes after the transcription start site of PnorV. 3 esaboxes false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520268 1 Spacer range2520268 1 346 353 annotation2520183 1 sigma54 range2520183 1 250 282 annotation2520179 1 norV promoter range2520179 1 1 285 annotation2520182 1 NorR binding 1 high affinity range2520182 1 189 209 annotation2520180 1 NorR binding 3 low affinity range2520180 1 149 158 annotation2520184 1 esabox range2520184 1 302 319 annotation2520186 1 esabox range2520186 1 328 345 annotation2520185 1 Spacer range2520185 1 320 327 annotation2520181 1 NorR binding 2 low affinity range2520181 1 162 178 annotation2520269 1 esabox range2520269 1 354 371 BBa_K2116076 1 BBa_K2116076 PnorV_(native)spacer_esabox_(8bp)spacer_esabox_(8bp)spacer_esabox_RBS_sfgfp(23) 2016-10-20T11:00:00Z 2016-10-25T12:02:01Z 23 23 false false _2584_ 31332 31336 9 false 23 false Asli Azizoglu component2529399 1 BBa_K2116006 component2529404 1 BBa_K2116017 component2529400 1 BBa_K2116090 annotation2529404 1 BBa_K2116017 range2529404 1 413 1138 annotation2529399 1 BBa_K2116006 range2529399 1 1 384 annotation2529400 1 BBa_K2116090 range2529400 1 385 412 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116076_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggtatcacgacctgtactatagtgcaggtaatccagcctgtactatagtgcaggggtacctctaaataaaagcgtataaataagaggaggttatcatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116006_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggtatcacgacctgtactatagtgcaggtaatccagcctgtactatagtgcaggggtacctctaaat BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z