LOCUS       BBa_K2116077            1076 bp    DNA     linear       22-Nov-2024
DEFINITION  PnorV_esabox_spacer_sfgfp
ACCESSION   BBa_K2116077
VERSION     BBa_K2116077.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=NorV promoter
     misc_feature    149..158
                     /label=NorR binding 3 (low affinity)
     misc_feature    162..178
                     /label=NorR binding 2 (low affinity)
     misc_feature    189..209
                     /label=NorR binding 1 (high affinity)
     misc_feature    292..309
                     /label=esabox
     misc_feature    250..282
                     /label=sigma54 binding site
     promoter        1..322
                     /label=BBa_K2116012
     misc_feature    323..350
                     /label=BBa_K2116090
     misc_feature    351..353
                     /label=ATG start codon
     misc_feature    1071..1076
                     /label=TAATAA stop codon
     CDS             351..1076
                     /label=sfGFP
     CDS             351..1076
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacggtac ccctgtacta
      301 tagtgcaggg gtacctctaa ataaaagcgt ataaataaga ggaggttatc atgagcaaag
      361 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      421 ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc
      481 ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc
      541 tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt
      601 tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg
      661 ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg
      721 agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgagtaca
      781 actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta
      841 acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac
      901 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac
      961 aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg
     1021 taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct taataa
//