LOCUS       BBa_K2116078            1102 bp    DNA     linear       10-Dec-2025
DEFINITION  PnorV_esabox_(8bp)spacer_esabox_spacer_sfgfp (25)
ACCESSION   BBa_K2116078
VERSION     BBa_K2116078.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=NorV promoter
     misc_feature    149..158
                     /label=NorR binding 3 (low affinity)
     misc_feature    162..178
                     /label=NorR binding 2 (low affinity)
     misc_feature    189..209
                     /label=NorR binding 1 (high affinity)
     misc_feature    250..282
                     /label=sigma54 binding site
     misc_feature    292..309
                     /label=esabox
     misc_feature    310..317
                     /label=spacer
     misc_feature    318..335
                     /label=esabox
     promoter        1..348
                     /label=BBa_K2116013
     misc_feature    349..376
                     /label=BBa_K2116090
     misc_feature    377..379
                     /label=ATG start codon
     misc_feature    1097..1102
                     /label=TAATAA stop codon
     CDS             377..1102
                     /label=sfGFP
     CDS             377..1102
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacggtac ccctgtacta
      301 tagtgcaggt agagtctcct gtactatagt gcaggggtac ctctaaataa aagcgtataa
      361 ataagaggag gttatcatga gcaaaggaga agaacttttc actggagttg tcccaattct
      421 tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg
      481 tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt
      541 tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc
      601 ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      661 acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga
      721 aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa
      781 cattcttgga cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga
      841 caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc
      901 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      961 accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg
     1021 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
     1081 gctctacaaa aggccttaat aa
//