BBa_K2116013 1 BBa_K2116013 AND gate p25 2016-10-13T11:00:00Z 2016-10-24T10:31:05Z PnorV from E.coli, esabox from []. AND gate constructed by adding an esabox that starts after the -12 binding site for sigma 54, and replaces some of the sigma 54 binding site. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2529027 1 spacer range2529027 1 310 317 annotation2520513 1 NorR binding 1 (high affinity) range2520513 1 189 209 annotation2520514 1 sigma54 binding site range2520514 1 250 282 annotation2520510 1 NorV promoter range2520510 1 1 285 annotation2520512 1 NorR binding 2 (low affinity) range2520512 1 162 178 annotation2520515 1 esabox range2520515 1 292 309 annotation2520511 1 NorR binding 3 (low affinity) range2520511 1 149 158 annotation2529028 1 esabox range2529028 1 318 335 BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520523 1 sfGFP range2520523 1 1 726 annotation2520522 1 TAATAA stop codon range2520522 1 721 726 annotation2520521 1 ATG start codon range2520521 1 1 3 BBa_K2116078 1 BBa_K2116078 PnorV_esabox_(8bp)spacer_esabox_spacer_sfgfp (25) 2016-10-20T11:00:00Z 2016-10-25T12:07:29Z 25 25 false false _2584_ 31332 31336 9 false 25 false Asli Azizoglu component2529488 1 BBa_K2116013 component2529489 1 BBa_K2116090 component2529493 1 BBa_K2116017 annotation2529489 1 BBa_K2116090 range2529489 1 349 376 annotation2529493 1 BBa_K2116017 range2529493 1 377 1102 annotation2529488 1 BBa_K2116013 range2529488 1 1 348 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116078_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacggtacccctgtactatagtgcaggtagagtctcctgtactatagtgcaggggtacctctaaataaaagcgtataaataagaggaggttatcatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc BBa_K2116013_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacggtacccctgtactatagtgcaggtagagtctcctgtactatagtgcaggggtacctctaaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z