LOCUS       BBa_K2116080            1119 bp    DNA     linear       01-May-2024
DEFINITION  PnorV_(native)spacer_esabox_(15bp)spacer_esabox_spacer_sfgfp(27)
ACCESSION   BBa_K2116080
VERSION     BBa_K2116080.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=norV promoter
     misc_feature    149..158
                     /label=NorR binding 3 low affinity
     misc_feature    162..178
                     /label=norR binding 2 low affinity
     misc_feature    189..209
                     /label=NorR binding 1 high affinity
     misc_feature    250..282
                     /label=sigma54
     misc_feature    302..319
                     /label=esabox
     misc_feature    320..334
                     /label=Spacer
     misc_feature    335..352
                     /label=esabox
     promoter        1..365
                     /label=BBa_K2116007
     misc_feature    366..393
                     /label=BBa_K2116090
     misc_feature    394..396
                     /label=ATG start codon
     misc_feature    1114..1119
                     /label=TAATAA stop codon
     CDS             394..1119
                     /label=sfGFP
     CDS             394..1119
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagggtac
      301 ccctgtacta tagtgcagga gcgtgatttc caatcctgta ctatagtgca ggggtacctc
      361 taaataaaag cgtataaata agaggaggtt atcatgagca aaggagaaga acttttcact
      421 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      481 cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt tatttgcact
      541 actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta tggtgttcaa
      601 tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc
      661 gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta caagacgcgt
      721 gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat
      781 tttaaagaag atggaaacat tcttggacac aaactcgagt acaactttaa ctcacacaat
      841 gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac
      901 aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc
      961 gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt cctttcgaaa
     1021 gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt
     1081 acacatggca tggatgagct ctacaaaagg ccttaataa
//