LOCUS       BBa_K2116081            1152 bp    DNA     linear       02-May-2024
DEFINITION  PnorV_(native)spacer_esabox_(15bp)spacer_esabox_(15bp)spacer_esabox
            _spacer_sfgfp(28)
ACCESSION   BBa_K2116081
VERSION     BBa_K2116081.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=NorV promoter
     misc_feature    149..158
                     /label=NorR binding 3 low affinity
     misc_feature    162..178
                     /label=NorR binding 2 low affinity
     misc_feature    189..209
                     /label=NorR binding 1 high affinity
     misc_feature    250..282
                     /label=sigma54
     misc_feature    302..319
                     /label=esabox
     misc_feature    320..334
                     /label=Spacer
     misc_feature    335..352
                     /label=esabox
     misc_feature    353..367
                     /label=Spacer
     misc_feature    368..385
                     /label=esabox
     promoter        1..398
                     /label=BBa_K2116008
     misc_feature    399..426
                     /label=BBa_K2116090
     misc_feature    427..429
                     /label=ATG start codon
     misc_feature    1147..1152
                     /label=TAATAA stop codon
     CDS             427..1152
                     /label=sfGFP
     CDS             427..1152
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagggtac
      301 ccctgtacta tagtgcagga gataccagat tgctcctgta ctatagtgca ggtactctgt
      361 aaggacacct gtactatagt gcaggggtac ctctaaataa aagcgtataa ataagaggag
      421 gttatcatga gcaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      481 gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg tgatgctaca
      541 aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt tccgtggcca
      601 acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc ggatcacatg
      661 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata
      721 tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga aggtgatacc
      781 cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa cattcttgga
      841 cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga caaacaaaag
      901 aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc cgttcaacta
      961 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1021 cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg tgaccacatg
     1081 gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa
     1141 aggccttaat aa
//