BBa_K2116008 1 BBa_K2116008 AND gate regulated by norR and esaR (three esaboxes,15bp) 2016-10-12T11:00:00Z 2016-10-25T12:23:56Z PnorV comes from E.coli. esabox [] AND gate constructed by placing three esaboxes after the transcription start site of PnorV. The esaboxes have 15bp of spacing in between. false false _2584_ 31332 31336 9 false ... false Asli Azizoglu annotation2520461 1 Spacer range2520461 1 320 334 annotation2520463 1 Spacer range2520463 1 353 367 annotation2520456 1 NorR binding 3 low affinity range2520456 1 149 158 annotation2520457 1 NorR binding 2 low affinity range2520457 1 162 178 annotation2520459 1 sigma54 range2520459 1 250 282 annotation2520460 1 esabox range2520460 1 302 319 annotation2520462 1 esabox range2520462 1 335 352 annotation2520464 1 esabox range2520464 1 368 385 annotation2520455 1 NorV promoter range2520455 1 1 285 annotation2520458 1 NorR binding 1 high affinity range2520458 1 189 209 BBa_K2116081 1 BBa_K2116081 PnorV_(native)spacer_esabox_(15bp)spacer_esabox_(15bp)spacer_esabox_spacer_sfgfp(28) 2016-10-20T11:00:00Z 2016-10-25T12:24:09Z asfg asfg false false _2584_ 31332 31336 9 false asfg false Asli Azizoglu component2529591 1 BBa_K2116017 component2529587 1 BBa_K2116090 component2529586 1 BBa_K2116008 annotation2529591 1 BBa_K2116017 range2529591 1 427 1152 annotation2529587 1 BBa_K2116090 range2529587 1 399 426 annotation2529586 1 BBa_K2116008 range2529586 1 1 398 BBa_K2116017 1 BBa_K2116017 super folder green fluorescent protein 2016-10-13T11:00:00Z 2016-10-14T07:40:00Z ? superfolder gfp false false _2584_ 31336 31336 9 false ... false Asli Azizoglu annotation2520523 1 sfGFP range2520523 1 1 726 annotation2520521 1 ATG start codon range2520521 1 1 3 annotation2520522 1 TAATAA stop codon range2520522 1 721 726 BBa_K2116090 1 BBa_K2116090 Spacer for BB assembly 2016-10-24T11:00:00Z 2016-10-24T11:15:10Z - This part is added to assemble our composite parts. Due to the repetitive nature of esaboxes, cloning of our parts containing PnorV promoter into the biobrick backbone make this step necessary. false false _2584_ 31332 31332 9 false - false Lukas Schmidheini BBa_K2116017_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116090_sequence 1 aaaagcgtataaataagaggaggttatc BBa_K2116081_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggagataccagattgctcctgtactatagtgcaggtactctgtaaggacacctgtactatagtgcaggggtacctctaaataaaagcgtataaataagaggaggttatcatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaaggccttaataa BBa_K2116008_sequence 1 acgtagcaacgcagacgcatcgcactccagcacctgacgtagcgtggtgatcaggcgctgaaaacgatcctggtgaccaatcccacgctgcaattcgatggcgatattcgccagcacatcaacggaaaaactcatctttgcctcactgtcaatttgactatagatattgtcatatcgaccatttgattgatagtcattttgactactcattaatgggcataattttatttatagagtaaaaacaatcagataaaaaactggcacgcaatctgcaattagcaagacatctttttagggtacccctgtactatagtgcaggagataccagattgctcctgtactatagtgcaggtactctgtaaggacacctgtactatagtgcaggggtacctctaaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z