LOCUS       BBa_K2116083            1142 bp    DNA     linear       22-Nov-2024
DEFINITION  PnorV_esabox_(15bp)spacer_esabox_(15bp)spacer_esabox_spacer_sfgfp(3
            0)
ACCESSION   BBa_K2116083
VERSION     BBa_K2116083.1  
FEATURES             Location/Qualifiers
     promoter        1..285
                     /label=NorV promoter
     misc_feature    149..158
                     /label=NorR binding 3 (low affinity)
     misc_feature    162..178
                     /label=NorR binding 2 (low affinity)
     misc_feature    189..209
                     /label=NorR binding 1 (low affinity)
     misc_feature    250..282
                     /label=sigma 54 binding site
     misc_feature    292..309
                     /label=esabox
     misc_feature    325..342
                     /label=esabox
     misc_feature    358..375
                     /label=esabox
     misc_feature    310..324
                     /label=spacer
     misc_feature    343..357
                     /label=spacer
     promoter        1..388
                     /label=BBa_K2116069
     misc_feature    389..416
                     /label=BBa_K2116090
     misc_feature    417..419
                     /label=ATG start codon
     misc_feature    1137..1142
                     /label=TAATAA stop codon
     CDS             417..1142
                     /label=sfGFP
     CDS             417..1142
                     /label=BBa_K2116017
ORIGIN
        1 acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg
       61 aaaacgatcc tggtgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
      121 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
      181 atttgattga tagtcatttt gactactcat taatgggcat aattttattt atagagtaaa
      241 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacggtac ccctgtacta
      301 tagtgcagga gataccagat tgctcctgta ctatagtgca ggtactctgt aaggacacct
      361 gtactatagt gcaggggtac ctctaaataa aagcgtataa ataagaggag gttatcatga
      421 gcaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      481 ttaatgggca caaattttct gtccgtggag agggtgaagg tgatgctaca aacggaaaac
      541 tcacccttaa atttatttgc actactggaa aactacctgt tccgtggcca acacttgtca
      601 ctactctgac ctatggtgtt caatgctttt cccgttatcc ggatcacatg aaacggcatg
      661 actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata tctttcaaag
      721 atgacgggac ctacaagacg cgtgctgaag tcaagtttga aggtgatacc cttgttaatc
      781 gtatcgagtt aaagggtatt gattttaaag aagatggaaa cattcttgga cacaaactcg
      841 agtacaactt taactcacac aatgtataca tcacggcaga caaacaaaag aatggaatca
      901 aagctaactt caaaattcgc cacaacgttg aagatggttc cgttcaacta gcagaccatt
      961 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1021 cgacacaatc tgtcctttcg aaagatccca acgaaaagcg tgaccacatg gtccttcttg
     1081 agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa aggccttaat
     1141 aa
//