LOCUS BBa_K2117009 4034 bp DNA linear 23-Dec-2024 DEFINITION Plasmid enabling Yarrowia lipolytica biobrick ACCESSION BBa_K2117009 VERSION BBa_K2117009.1 FEATURES Location/Qualifiers primer_bind 1..21 /label=BioBrick suffix primer_bind 14..43 /label=SB-prep-pSB1A8YL-fw stem_loop 22..273 /label=CYC1 terminator primer_bind 271..290 /label=140 misc_feature 327..427 /label=Cen1-1 origin of replication CDS 595..1455 /label=URA3 (uracil selection marker) misc_feature 1246..1246 /label=C to A misc_feature 1405..1405 /label=T to C misc_feature 1438..1438 /label=C to G promoter 1456..1540 /label=pURA3 misc_feature 1496..1496 /label=C to G misc_feature 1693..1806 /label=ORI1001 origin of replication promoter 2175..2203 /label=pAmpR CDS 2245..3105 /label=AmpR (ampicillin selection marker) misc_feature 3260..3879 /label=pBR322 origin of replication primer_bind 3993..4012 /label=VR primer_bind 3997..4020 /label=SB-prep-pSB1A8YL-re misc_feature 4014..4034 /label=BioBrick prefix ORIGIN 1 tactagtagc ggccgctgca gcatgtaatt agttatgtca cgcttacatt cacgccctcc 61 ctccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat 121 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt 181 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt 241 tttgggacgc tcgaaggctt taatttgcaa gcttgatacc gctcgccgca gccgaacgac 301 cgagcgcagc gagtgcgccc aatacgatat cttaccaaat taggtaatct gcaaaagttc 361 agactgtgaa atgtaacatt ttatatatca agctctattt aacgcctcac agtagttaac 421 ataaagacaa accgcctctc cccgcgcgtt ggccgattca ttaatgcgca ggttgtgcag 481 tatcatacat actcgatcag acaggtcgtc tgaccatcat acaagctgaa caagcgctcc 541 atacttgcac gctctctata tacacagtta aatgacatat ccatagtcta acctctaaca 601 gttaatcttc tggtaagcct cccagccagc cttctggtat cgcttggcct cctcaatagg 661 atctcggttc tggccgtaca gacctcggcc gacaattatg atatccgttc cggtagacat 721 gacatcctca acagttcggt actgctgtcc gagagcgtct cccttgtcgt caagacccac 781 cccgggggtc agaataagcc agtcctcaga gtcgccctta ggtcggttct gggcaatgaa 841 gccaaccaca aactcggggt cggatcgggc aagctcaatg gtctgcttgg agtactcgcc 901 agtggccaga gagcccttgc aagacagctc ggccagcatg agcagacctc tggccagctt 961 ctcgttggga gaggggacca ggaactcctt gtactgggag ttctcgtagt cagagacgtc 1021 ctccttcttc tgttcagaga cagtttcctc ggcaccagct cgcaggccag caatgattcc 1081 ggttccgggt acaccgtggg cgttggtgat atcggaccac tcggcgattc ggtagacacc 1141 gttcttgtac tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag 1201 gaagaaaccg tgcttaagag caagttcctt gagggggagc acagttccgg cgtaggtgaa 1261 gtcgtcaatg atgtcgatat gggtcttgat catgcacaca taaggtccga ccttatcggc 1321 aagctcaatg agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt 1381 ggctgccacg agcttgagca ctcgggcggc aaaggcggac ttgtggacgt tagctcgcgc 1441 ttcgtaggag ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcacaact 1501 ttttatcgga accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag 1561 ttgaacttat agatagactg gactatacgg ctatcggtcc aaattagaaa acaggtttcc 1621 cgactggaaa gcgggcagtg agcgcaacgg gacccaaacc ttgtcgatgc cgatagcgct 1681 atcgaacgta cccatactac tgtatattca agcaagtata tccgtgggtg cgggtgattt 1741 ggatctaagg ttcgtactca acactcacga gcagcttgcc tatgttacat ccttttatca 1801 gacatatctt gtatatatac gcacttttgc ccgtgctatg tcggtatttc acaccgcata 1861 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 1921 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 1981 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 2041 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 2101 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 2161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 2221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 2281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 2341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 2401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 2461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 2521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 2581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 2641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 2701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 2761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 2821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 2881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 2941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 3001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 3061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 3121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 3181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 3241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 3301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 3361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 3421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 3481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 3541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 3601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 3661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 3721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 3781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 3841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 3901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 3961 cggcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gcgaattcgc 4021 ggccgcttct agag //