##gff-version 3
##sequence-region BBa_K2117009 1 4034
BBa_K2117009	.	promoter	1456	1540	.	+	0	ID=annotation2516567;Name=pURA3
BBa_K2117009	.	sequence_alteration	1405	1405	.	+	0	ID=annotation2516565;Name=T to C
BBa_K2117009	.	primer_binding_site	1	21	.	+	0	ID=annotation2516558;Name=BioBrick suffix
BBa_K2117009	.	primer_binding_site	3997	4020	.	+	0	ID=annotation2516574;Name=SB-prep-pSB1A8YL-re
BBa_K2117009	.	CDS	2245	3105	.	+	0	ID=annotation2516571;Name=AmpR (ampicillin selection marker)
BBa_K2117009	.	promoter	2175	2203	.	+	0	ID=annotation2516570;Name=pAmpR
BBa_K2117009	.	primer_binding_site	271	290	.	+	0	ID=annotation2516561;Name=140
BBa_K2117009	.	sequence_feature	1693	1806	.	+	0	ID=annotation2516569;Name=ORI1001 origin of replication
BBa_K2117009	.	sequence_alteration	1246	1246	.	+	0	ID=annotation2516564;Name=C to A
BBa_K2117009	.	sequence_feature	327	427	.	+	0	ID=annotation2516562;Name=Cen1-1 origin of replication
BBa_K2117009	.	stem_loop	22	273	.	+	0	ID=annotation2516560;Name=CYC1 terminator
BBa_K2117009	.	sequence_feature	4014	4034	.	+	0	ID=annotation2516575;Name=BioBrick prefix
BBa_K2117009	.	sequence_alteration	1496	1496	.	+	0	ID=annotation2516568;Name=C to G
BBa_K2117009	.	primer_binding_site	3993	4012	.	+	0	ID=annotation2516573;Name=VR
BBa_K2117009	.	sequence_feature	3260	3879	.	+	0	ID=annotation2516572;Name=pBR322 origin of replication
BBa_K2117009	.	CDS	595	1455	.	+	0	ID=annotation2516563;Name=URA3 (uracil selection marker)
BBa_K2117009	.	sequence_alteration	1438	1438	.	+	0	ID=annotation2516566;Name=C to G
BBa_K2117009	.	primer_binding_site	14	43	.	+	0	ID=annotation2516559;Name=SB-prep-pSB1A8YL-fw
>BBa_K2117009
tactagtagcggccgctgcagcatgtaattagttatgtcacgcttacattcacgccctccctccacatccgctctaacc
gaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttat
ttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaa
ggttttgggacgctcgaaggctttaatttgcaagcttgataccgctcgccgcagccgaacgaccgagcgcagcgagtgc
gcccaatacgatatcttaccaaattaggtaatctgcaaaagttcagactgtgaaatgtaacattttatatatcaagctc
tatttaacgcctcacagtagttaacataaagacaaaccgcctctccccgcgcgttggccgattcattaatgcgcaggtt
gtgcagtatcatacatactcgatcagacaggtcgtctgaccatcatacaagctgaacaagcgctccatacttgcacgct
ctctatatacacagttaaatgacatatccatagtctaacctctaacagttaatcttctggtaagcctcccagccagcct
tctggtatcgcttggcctcctcaataggatctcggttctggccgtacagacctcggccgacaattatgatatccgttcc
ggtagacatgacatcctcaacagttcggtactgctgtccgagagcgtctcccttgtcgtcaagacccaccccgggggtc
agaataagccagtcctcagagtcgcccttaggtcggttctgggcaatgaagccaaccacaaactcggggtcggatcggg
caagctcaatggtctgcttggagtactcgccagtggccagagagcccttgcaagacagctcggccagcatgagcagacc
tctggccagcttctcgttgggagaggggaccaggaactccttgtactgggagttctcgtagtcagagacgtcctccttc
ttctgttcagagacagtttcctcggcaccagctcgcaggccagcaatgattccggttccgggtacaccgtgggcgttgg
tgatatcggaccactcggcgattcggtagacaccgttcttgtactggtgcttgacagtgttgccaatatctgcgaactt
tctgtcctcgaacaggaagaaaccgtgcttaagagcaagttccttgagggggagcacagttccggcgtaggtgaagtcg
tcaatgatgtcgatatgggtcttgatcatgcacacataaggtccgaccttatcggcaagctcaatgagctccttggtgg
tggtaacatccagagaagcacacaggttggttttcttggctgccacgagcttgagcactcgggcggcaaaggcggactt
gtggacgttagctcgcgcttcgtaggagggcattttggtggtgaagaggagactgaaataaatttagtctgcacaactt
tttatcggaaccttatctggggcagtgaagtatatgttatggtaatagttacgagttagttgaacttatagatagactg
gactatacggctatcggtccaaattagaaaacaggtttcccgactggaaagcgggcagtgagcgcaacgggacccaaac
cttgtcgatgccgatagcgctatcgaacgtacccatactactgtatattcaagcaagtatatccgtgggtgcgggtgat
ttggatctaaggttcgtactcaacactcacgagcagcttgcctatgttacatccttttatcagacatatcttgtatata
tacgcacttttgcccgtgctatgtcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgca
tagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttaca
gacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaaggg
cctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcgggga
aatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgat
aaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggc
attttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtg
ggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagca
cttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacacta
ttctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgc
agtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccg
cttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacga
cgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagct
tcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggct
ggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagcc
ctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggt
gcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcattttt
aatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactg
agcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaaca
aaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttca
gcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcc
tacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactca
agacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacga
cctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggta
tccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcct
gtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgcca
gcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattc
tgtggataaccggcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagcgaattcgcggccgcttc
tagag