BBa_K212004
1
BBa_K212004
secretion signal peptide + GFPmut3b
2009-10-19T11:00:00Z
2015-05-08T01:11:29Z
This part was synthesized based on a known sequence, which may be found here: .
This is a composite signalling part. The secretion signal peptide for beta-lactamase in S. epidermidis is ligated N-terminally to registry part BBa_E0040 (GFPmut3b). The signal peptide motif is used by the bacteria to target the protein for secretion through the Sec pathway. Any part may be put in place of GFP, but in its current state, the amount of secretion of any protein may be studied using confocal microscopy.
NOTE: This part only works for S. epidermidis ATCC 12228
false
false
_314_
0
4681
9
It's complicated
true
Not Applicable.
false
Elias Scheer
annotation2061378
1
GFPmut3b
range2061378
1
118
836
annotation2061374
1
secretion signal peptide
range2061374
1
23
111
BBa_K212004_sequence
1
gaattcgcggccgcttctagaatgaaaaagttaatatttttaattgtaattgctttagttttaagtgcatgtaattcaaacagttcacatgccaaagagttaaatgatttatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z