LOCUS       BBa_K2120301             919 bp    DNA     linear       26-Nov-2024
DEFINITION  pR+B0032+RFP+B0015
ACCESSION   BBa_K2120301
VERSION     BBa_K2120301.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..70
                     /label=RBS-3\Weak
     RBS             64..67
                     /label=RBS
     misc_feature    58..70
                     /label=BBa_B0032
     RBS             58..70
                     /label=BBa_B0032
     CDS             77..751
                     /label=mrfp1
     misc_feature    758..782
                     /label=Help:Barcodes
     CDS             77..782
                     /label=BBa_E1010
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
     terminator      791..919
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaag tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
      121 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      181 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      241 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      301 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      361 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      421 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
      481 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      541 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
      601 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
      661 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
      721 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
      781 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttata
//