LOCUS       BBa_K2120303            2057 bp    DNA     linear       26-Dec-2024
DEFINITION  araC-pBAD+B0034+tetR+B0015
ACCESSION   BBa_K2120303
VERSION     BBa_K2120303.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     misc_feature    1222..1225
                     /label=conserved
     RBS             1218..1229
                     /label=BBa_B0034
     CDS             1239..1855
                     /label=tetR
     misc_feature    1856..1889
                     /label=SsrA
     misc_feature    1896..1920
                     /label=Help:Barcodes
     CDS             1236..1920
                     /label=BBa_C0040
     stem_loop       1940..1983
                     /label=stem_loop
     misc_feature    1929..2008
                     /label=BBa_B0010
     terminator      1929..2008
                     /label=BBa_B0010
     stem_loop       2024..2043
                     /label=T7 TE
     misc_feature    2050..2050
                     /label=stop
     polyA_site      2044..2057
                     /label=polya
     misc_feature    2017..2057
                     /label=BBa_B0012
     terminator      2017..2057
                     /label=BBa_B0012
     terminator      1929..2057
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag
     1261 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
     1321 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
     1381 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
     1441 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
     1501 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
     1561 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
     1621 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
     1681 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
     1741 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
     1801 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
     1861 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     1921 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2041 gcctttctgc gtttata
//