LOCUS       BBa_K2120306            2286 bp    DNA     linear       22-Nov-2024
DEFINITION  B0015+CI+B0034+pBAD-AraC+B0015
ACCESSION   BBa_K2120306
VERSION     BBa_K2120306.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..912
                     /label=BBa_K2120105
     misc_feature    921..932
                     /label=inverse RBS
     RBS             921..932
                     /label=BBa_K1045010
     stem_loop       2169..2212
                     /label=stem_loop
     misc_feature    2158..2237
                     /label=BBa_B0010
     terminator      2158..2237
                     /label=BBa_B0010
     stem_loop       2253..2272
                     /label=T7 TE
     misc_feature    2279..2279
                     /label=stop
     polyA_site      2273..2286
                     /label=polya
     misc_feature    2246..2286
                     /label=BBa_B0012
     terminator      2246..2286
                     /label=BBa_B0012
     terminator      2158..2286
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt
      241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg
      301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag
      361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat
      421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc
      481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc
      541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg
      601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat
      661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa
      721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg
      781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt
      841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt
      901 ttttgtgctc attactagag tttctcctct tttactagag gttagcccaa aaaacgggta
      961 tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga
     1021 taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct
     1081 gccgtgatta tagacacttt tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta
     1141 cagaatgctt ttaataagcg gggttaccgg tttggttagc gagaagagcc agtaaaagac
     1201 gcagtgacgg caatgtctga tgcaatatgg acaattggtt tcttctctga atggcgggag
     1261 tatgaaaagt atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc
     1321 ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga
     1381 ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt
     1441 gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg
     1501 agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta
     1561 ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac
     1621 ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat
     1681 cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga
     1741 gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa
     1801 tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat
     1861 cgccagcgtc gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca
     1921 gcagttaggg attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct
     1981 gcttttgagc accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga
     2041 tcaactctat ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg
     2101 tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat actagagcca
     2161 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2221 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2281 tttata
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