LOCUS BBa_K2120308 3415 bp DNA linear 22-Dec-2024 DEFINITION B0015+LacIq+B0034+pBAD-AraC+plac+B0034+eYFP+B0015 ACCESSION BBa_K2120308 VERSION BBa_K2120308.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 CDS 138..1229 /label=BBa_K2120107 misc_feature 1238..1249 /label=inverse RBS RBS 1238..1249 /label=BBa_K1045010 promoter 1258..2466 /label=promoter promoter 1258..2466 /label=BBa_K2120100 misc_feature 2477..2493 /label=lac O1 promoter 2494..2499 /label=-35 misc_feature 2500..2516 /label=lac O1 promoter 2517..2522 /label=-10 misc_feature 2475..2528 /label=BBa_R0011 promoter 2475..2528 /label=BBa_R0011 misc_feature 2542..2545 /label=conserved RBS 2538..2549 /label=BBa_B0034 CDS 2556..3278 /label=BBa_E0030 stem_loop 3298..3341 /label=stem_loop misc_feature 3287..3366 /label=BBa_B0010 terminator 3287..3366 /label=BBa_B0010 stem_loop 3382..3401 /label=T7 TE misc_feature 3408..3408 /label=stop polyA_site 3402..3415 /label=polya misc_feature 3375..3415 /label=BBa_B0012 terminator 3375..3415 /label=BBa_B0012 terminator 3287..3415 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagagtca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 181 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt 241 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag 301 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg 361 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata 421 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga 481 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt 541 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg 601 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg 661 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc 721 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga 781 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc 841 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta 901 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg 961 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg 1021 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg 1081 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg 1141 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg 1201 tataacgtta ctggtttcac attcaccact actagagttt ctcctctttt actagaggtt 1261 agcccaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg tcaggtagga 1321 tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa 1381 tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt tgtcatggct 1441 ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt ggttagcgag 1501 aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca attggtttct 1561 tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg 1621 ggatactcgt ttaatgccca tctggtggcg ggtttaacgc cgattgaggc caacggttat 1681 ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt 1741 cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tttgccgacc gggtgatatt 1801 ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg 1861 tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg 1921 tcaatatttg ccaatacggg gttctttcgc ccggatgaag cgcaccagcc gcatttcagc 1981 gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc ggagctgctg 2041 gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa cgagtcgctc 2101 catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga tcacctggca 2161 gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc gtcgcgtctg 2221 tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga ggaccaacgt 2281 atcagccagg cgaagctgct tttgagcacc acccggatgc ctatcgccac cgtcggtcgc 2341 aatgttggtt ttgacgatca actctatttc tcgcgggtat ttaaaaaatg caccggggcc 2401 agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg 2461 tcataatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2521 ctgagcacat actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 2581 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2641 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2701 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc 2761 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca 2821 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2881 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2941 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 3001 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 3061 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 3121 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga 3181 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 3241 ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata 3301 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3361 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //