LOCUS       BBa_K2120309            3076 bp    DNA     linear       22-Dec-2024
DEFINITION  B0015+CI+B0034+pBAD-AraC+pR+B0032+RFP+B0015
ACCESSION   BBa_K2120309
VERSION     BBa_K2120309.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..912
                     /label=BBa_K2120105
     misc_feature    921..932
                     /label=inverse RBS
     RBS             921..932
                     /label=BBa_K1045010
     promoter        2195..2200
                     /label=-10
     promoter        2172..2177
                     /label=-35
     misc_feature    2182..2198
                     /label=OR1
     misc_feature    2158..2174
                     /label=OR2
     misc_feature    2158..2206
                     /label=BBa_R0051
     promoter        2158..2206
                     /label=BBa_R0051
     RBS             2215..2227
                     /label=RBS-3\Weak
     RBS             2221..2224
                     /label=RBS
     misc_feature    2215..2227
                     /label=BBa_B0032
     RBS             2215..2227
                     /label=BBa_B0032
     CDS             2234..2908
                     /label=mrfp1
     misc_feature    2915..2939
                     /label=Help:Barcodes
     CDS             2234..2939
                     /label=BBa_E1010
     stem_loop       2959..3002
                     /label=stem_loop
     misc_feature    2948..3027
                     /label=BBa_B0010
     terminator      2948..3027
                     /label=BBa_B0010
     stem_loop       3043..3062
                     /label=T7 TE
     misc_feature    3069..3069
                     /label=stop
     polyA_site      3063..3076
                     /label=polya
     misc_feature    3036..3076
                     /label=BBa_B0012
     terminator      3036..3076
                     /label=BBa_B0012
     terminator      2948..3076
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt
      241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg
      301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag
      361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat
      421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc
      481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc
      541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg
      601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat
      661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa
      721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg
      781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt
      841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt
      901 ttttgtgctc attactagag tttctcctct tttactagag gttagcccaa aaaacgggta
      961 tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga
     1021 taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct
     1081 gccgtgatta tagacacttt tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta
     1141 cagaatgctt ttaataagcg gggttaccgg tttggttagc gagaagagcc agtaaaagac
     1201 gcagtgacgg caatgtctga tgcaatatgg acaattggtt tcttctctga atggcgggag
     1261 tatgaaaagt atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc
     1321 ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga
     1381 ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt
     1441 gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg
     1501 agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta
     1561 ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac
     1621 ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat
     1681 cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga
     1741 gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa
     1801 tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat
     1861 cgccagcgtc gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca
     1921 gcagttaggg attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct
     1981 gcttttgagc accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga
     2041 tcaactctat ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg
     2101 tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat actagagtaa
     2161 caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact agagtcacac
     2221 aggaaagtac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
     2281 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
     2341 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
     2401 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     2461 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
     2521 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
     2581 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     2641 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     2701 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     2761 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     2821 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     2881 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
     2941 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3001 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     3061 cctttctgcg tttata
//