##gff-version 3
##sequence-region BBa_K2120309 1 3076
BBa_K2120309	.	ribosome_entry_site	921	932	.	+	0	ID=BBa_K1045010;Name=BBa_K1045010
BBa_K2120309	.	non_covalent_binding_site	921	932	.	+	0	ID=annotation2360435;Name=inverse RBS;Parent=BBa_K1045010
BBa_K2120309	.	ribosome_entry_site	2215	2227	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2120309	.	engineered_region	2215	2227	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2120309	.	ribosome_entry_site	2215	2227	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2120309	.	ribosome_entry_site	2221	2224	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2120309	.	terminator	1	129	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_K2120309	.	engineered_region	1	41	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_K2120309	.	engineered_region	50	129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_K2120309	.	promoter	2158	2206	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K2120309	.	non_covalent_binding_site	2182	2198	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K2120309	.	engineered_region	2158	2206	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K2120309	.	non_covalent_binding_site	2158	2174	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K2120309	.	promoter	2172	2177	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K2120309	.	promoter	2195	2200	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K2120309	.	CDS	2234	2939	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2120309	.	CDS	2234	2908	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2120309	.	engineered_tag	2915	2939	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2120309	.	CDS	138	912	.	+	0	ID=BBa_K2120105;Name=BBa_K2120105
BBa_K2120309	.	terminator	2948	3076	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2120309	.	terminator	3036	3076	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2120309	.	polyA_site	3063	3076	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2120309	.	engineered_region	3036	3076	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2120309	.	stem_loop	3043	3062	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2120309	.	stop_codon	3069	3069	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2120309	.	terminator	2948	3027	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2120309	.	stem_loop	2959	3002	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2120309	.	engineered_region	2948	3027	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K2120309
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcgatctacactagcactatc
agcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataac
tttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccg
ctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcct
gctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttc
aacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggtt
ctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgca
tactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttt
tgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatcccc
atcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgt
cctcaagctgctcttgtgttaatggtttcttttttgtgctcattactagagtttctcctcttttactagaggttagccc
aaaaaacgggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaat
gctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttac
gcgtttttgtcatggctttggtcccgctttgttacagaatgcttttaataagcggggttaccggtttggttagcgagaa
gagccagtaaaagacgcagtgacggcaatgtctgatgcaatatggacaattggtttcttctctgaatggcgggagtatg
aaaagtatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaatgcccatctggtggcgggtttaacgc
cgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccat
tcgcggtcagggggtggtgaaaaatcagggacgagaatttgtttgccgaccgggtgatattttgctgttcccgccagga
gagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctact
ggcatgaatggcttaactggccgtcaatatttgccaatacggggttctttcgcccggatgaagcgcaccagccgcattt
cagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgctt
gagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggctt
gtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtc
gcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgtatcagccaggcg
aagctgcttttgagcaccacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttct
cgcgggtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagc
cgtcaagttgtcataatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactag
agtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaag
gttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaact
gaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttac
gttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaact
tcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcg
tggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtac
ccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagtta
aaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctccca
caacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatag
tgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata