LOCUS BBa_K2120310 3213 bp DNA linear 22-Dec-2024 DEFINITION B0015+CI+B0034+pBAD-araC+B0015+pR+B0032+RFP+B0015 ACCESSION BBa_K2120310 VERSION BBa_K2120310.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 CDS 138..912 /label=BBa_K2120105 misc_feature 925..928 /label=conserved RBS 921..932 /label=BBa_B0034 promoter 941..2149 /label=promoter promoter 941..2149 /label=BBa_K2120100 stem_loop 2169..2212 /label=stem_loop misc_feature 2158..2237 /label=BBa_B0010 terminator 2158..2237 /label=BBa_B0010 stem_loop 2253..2272 /label=T7 TE misc_feature 2279..2279 /label=stop polyA_site 2273..2286 /label=polya misc_feature 2246..2286 /label=BBa_B0012 terminator 2246..2286 /label=BBa_B0012 terminator 2158..2286 /label=BBa_B0015 promoter 2332..2337 /label=-10 promoter 2309..2314 /label=-35 misc_feature 2319..2335 /label=OR1 misc_feature 2295..2311 /label=OR2 misc_feature 2295..2343 /label=BBa_R0051 promoter 2295..2343 /label=BBa_R0051 RBS 2352..2364 /label=RBS-3\Weak RBS 2358..2361 /label=RBS misc_feature 2352..2364 /label=BBa_B0032 RBS 2352..2364 /label=BBa_B0032 CDS 2371..3045 /label=mrfp1 misc_feature 3052..3076 /label=Help:Barcodes CDS 2371..3076 /label=BBa_E1010 stem_loop 3096..3139 /label=stem_loop misc_feature 3085..3164 /label=BBa_B0010 terminator 3085..3164 /label=BBa_B0010 stem_loop 3180..3199 /label=T7 TE misc_feature 3206..3206 /label=stop polyA_site 3200..3213 /label=polya misc_feature 3173..3213 /label=BBa_B0012 terminator 3173..3213 /label=BBa_B0012 terminator 3085..3213 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt 241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg 301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag 361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat 421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc 481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc 541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg 601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat 661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa 721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg 781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt 841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt 901 ttttgtgctc attactagag aaagaggaga aatactagag gttagcccaa aaaacgggta 961 tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga 1021 taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct 1081 gccgtgatta tagacacttt tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta 1141 cagaatgctt ttaataagcg gggttaccgg tttggttagc gagaagagcc agtaaaagac 1201 gcagtgacgg caatgtctga tgcaatatgg acaattggtt tcttctctga atggcgggag 1261 tatgaaaagt atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc 1321 ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga 1381 ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt 1441 gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg 1501 agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta 1561 ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac 1621 ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat 1681 cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga 1741 gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa 1801 tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat 1861 cgccagcgtc gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca 1921 gcagttaggg attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct 1981 gcttttgagc accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga 2041 tcaactctat ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg 2101 tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat actagagcca 2161 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2221 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2281 tttatatact agagtaacac cgtgcgtgtt gactatttta cctctggcgg tgataatggt 2341 tgctactaga gtcacacagg aaagtactag atggcttcct ccgaagacgt tatcaaagag 2401 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa 2461 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa 2521 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa 2581 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt 2641 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac 2701 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg 2761 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg 2821 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt 2881 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg 2941 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc 3001 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag 3061 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3121 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 3181 gctcaccttc gggtgggcct ttctgcgttt ata //