##gff-version 3
##sequence-region BBa_K2120310 1 3213
BBa_K2120310	.	ribosome_entry_site	921	932	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2120310	.	conserved_region	925	928	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2120310	.	CDS	2371	3076	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2120310	.	engineered_tag	3052	3076	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2120310	.	CDS	2371	3045	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2120310	.	terminator	3085	3213	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2120310	.	terminator	3085	3164	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2120310	.	engineered_region	3085	3164	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2120310	.	stem_loop	3096	3139	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2120310	.	terminator	3173	3213	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2120310	.	stop_codon	3206	3206	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2120310	.	polyA_site	3200	3213	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2120310	.	stem_loop	3180	3199	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2120310	.	engineered_region	3173	3213	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2120310	.	CDS	138	912	.	+	0	ID=BBa_K2120105;Name=BBa_K2120105
BBa_K2120310	.	promoter	941	2149	.	+	0	ID=BBa_K2120100;Name=BBa_K2120100
BBa_K2120310	.	promoter	941	2149	.	+	0	ID=annotation2525146;Name=promoter;Parent=BBa_K2120100
BBa_K2120310	.	ribosome_entry_site	2352	2364	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2120310	.	ribosome_entry_site	2352	2364	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2120310	.	engineered_region	2352	2364	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2120310	.	ribosome_entry_site	2358	2361	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2120310	.	terminator	1	129	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_K2120310	.	engineered_region	1	41	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_K2120310	.	engineered_region	50	129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_K2120310	.	promoter	2295	2343	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K2120310	.	engineered_region	2295	2343	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K2120310	.	promoter	2332	2337	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K2120310	.	non_covalent_binding_site	2319	2335	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K2120310	.	promoter	2309	2314	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K2120310	.	non_covalent_binding_site	2295	2311	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K2120310	.	terminator	2158	2286	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2120310	.	terminator	2158	2237	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2120310	.	engineered_region	2158	2237	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2120310	.	stem_loop	2169	2212	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2120310	.	terminator	2246	2286	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2120310	.	stop_codon	2279	2279	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2120310	.	polyA_site	2273	2286	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2120310	.	stem_loop	2253	2272	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2120310	.	engineered_region	2246	2286	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K2120310
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcgatctacactagcactatc
agcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataac
tttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccg
ctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcct
gctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttc
aacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggtt
ctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgca
tactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttt
tgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatcccc
atcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgt
cctcaagctgctcttgtgttaatggtttcttttttgtgctcattactagagaaagaggagaaatactagaggttagccc
aaaaaacgggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaat
gctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttac
gcgtttttgtcatggctttggtcccgctttgttacagaatgcttttaataagcggggttaccggtttggttagcgagaa
gagccagtaaaagacgcagtgacggcaatgtctgatgcaatatggacaattggtttcttctctgaatggcgggagtatg
aaaagtatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaatgcccatctggtggcgggtttaacgc
cgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccat
tcgcggtcagggggtggtgaaaaatcagggacgagaatttgtttgccgaccgggtgatattttgctgttcccgccagga
gagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctact
ggcatgaatggcttaactggccgtcaatatttgccaatacggggttctttcgcccggatgaagcgcaccagccgcattt
cagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgctt
gagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggctt
gtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtc
gcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgtatcagccaggcg
aagctgcttttgagcaccacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttct
cgcgggtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagc
cgtcaagttgtcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactag
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata