LOCUS       BBa_K2120311            3127 bp    DNA     linear       22-Nov-2024
DEFINITION  B0015+tetR+B0034+pBAD-AraC+B0015+ptet+B0034+RFP+B0015
ACCESSION   BBa_K2120311
VERSION     BBa_K2120311.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     promoter        138..1346
                     /label=promoter
     promoter        138..822
                     /label=BBa_K2120100
     misc_feature    831..842
                     /label=inverse RBS
     RBS             831..842
                     /label=BBa_K1045010
     stem_loop       2079..2122
                     /label=stem_loop
     misc_feature    2068..2147
                     /label=BBa_B0010
     terminator      2068..2147
                     /label=BBa_B0010
     stem_loop       2163..2182
                     /label=T7 TE
     misc_feature    2189..2189
                     /label=stop
     polyA_site      2183..2196
                     /label=polya
     misc_feature    2156..2196
                     /label=BBa_B0012
     terminator      2156..2196
                     /label=BBa_B0012
     terminator      2068..2196
                     /label=BBa_B0015
     misc_feature    2205..2223
                     /label=TetR 1
     misc_feature    2205..2258
                     /label=BBa_R0040
     promoter        2224..2229
                     /label=-35
     misc_feature    2230..2248
                     /label=TetR 2
     promoter        2247..2252
                     /label=-10
     promoter        2205..2258
                     /label=BBa_R0040
     misc_feature    2271..2274
                     /label=conserved
     RBS             2267..2278
                     /label=BBa_B0034
     CDS             2285..2959
                     /label=mrfp1
     misc_feature    2966..2990
                     /label=Help:Barcodes
     CDS             2285..2990
                     /label=BBa_E1010
     stem_loop       3010..3053
                     /label=stem_loop
     misc_feature    2999..3078
                     /label=BBa_B0010
     terminator      2999..3078
                     /label=BBa_B0010
     stem_loop       3094..3113
                     /label=T7 TE
     misc_feature    3120..3120
                     /label=stop
     polyA_site      3114..3127
                     /label=polya
     misc_feature    3087..3127
                     /label=BBa_B0012
     terminator      3087..3127
                     /label=BBa_B0012
     terminator      2999..3127
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggtg atctacacta gcactatcag tgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cggacccact ttcacattta agttgttttt ctaatccgca
      241 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      301 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      361 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
      421 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
      481 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
      541 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
      601 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
      661 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
      721 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
      781 taatgcgctg ttaatcactt tacttttatc taatctggac attactagag tttctcctct
      841 tttactagag gttagcccaa aaaacgggta tggagaaaca gtagagagtt gcgataaaaa
      901 gcgtcaggta ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac
      961 gccgtgcaaa taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt
     1021 ttttgtcatg gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg
     1081 tttggttagc gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg
     1141 acaattggtt tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga
     1201 tcccctgctg ccgggatact cgtttaatgc ccatctggtg gcgggtttaa cgccgattga
     1261 ggccaacggt tatctcgatt tttttatcga ccgaccgctg ggaatgaaag gttatattct
     1321 caatctcacc attcgcggtc agggggtggt gaaaaatcag ggacgagaat ttgtttgccg
     1381 accgggtgat attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga
     1441 ggctcgcgaa tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg
     1501 gcttaactgg ccgtcaatat ttgccaatac ggggttcttt cgcccggatg aagcgcacca
     1561 gccgcatttc agcgacctgt ttgggcaaat cattaacgcc gggcaagggg aagggcgcta
     1621 ttcggagctg ctggcgataa atctgcttga gcaattgtta ctgcggcgca tggaagcgat
     1681 taacgagtcg ctccatccac cgatggataa tcgggtacgc gaggcttgtc agtacatcag
     1741 cgatcacctg gcagacagca attttgatat cgccagcgtc gcacagcatg tttgcttgtc
     1801 gccgtcgcgt ctgtcacatc ttttccgcca gcagttaggg attagcgtct taagctggcg
     1861 cgaggaccaa cgtatcagcc aggcgaagct gcttttgagc accacccgga tgcctatcgc
     1921 caccgtcggt cgcaatgttg gttttgacga tcaactctat ttctcgcggg tatttaaaaa
     1981 atgcaccggg gccagcccga gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt
     2041 agccgtcaag ttgtcataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2101 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2161 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag
     2221 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata
     2281 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
     2341 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     2401 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     2461 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     2521 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     2581 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     2641 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     2701 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     2761 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     2821 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     2881 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     2941 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc
     3001 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3061 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     3121 gtttata
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