##gff-version 3
##sequence-region BBa_K2120311 1 3127
BBa_K2120311	.	terminator	2999	3127	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2120311	.	terminator	2999	3078	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2120311	.	stem_loop	3010	3053	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2120311	.	engineered_region	2999	3078	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2120311	.	terminator	3087	3127	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2120311	.	stop_codon	3120	3120	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2120311	.	engineered_region	3087	3127	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2120311	.	stem_loop	3094	3113	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2120311	.	polyA_site	3114	3127	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2120311	.	ribosome_entry_site	2267	2278	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2120311	.	conserved_region	2271	2274	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2120311	.	terminator	1	129	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_K2120311	.	engineered_region	1	41	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_K2120311	.	engineered_region	50	129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_K2120311	.	CDS	2285	2990	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2120311	.	engineered_tag	2966	2990	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2120311	.	CDS	2285	2959	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2120311	.	promoter	2205	2258	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2120311	.	promoter	2224	2229	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2120311	.	non_covalent_binding_site	2230	2248	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2120311	.	engineered_region	2205	2258	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2120311	.	non_covalent_binding_site	2205	2223	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2120311	.	promoter	2247	2252	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2120311	.	terminator	2068	2196	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2120311	.	terminator	2068	2147	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2120311	.	stem_loop	2079	2122	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2120311	.	engineered_region	2068	2147	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2120311	.	terminator	2156	2196	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2120311	.	stop_codon	2189	2189	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2120311	.	engineered_region	2156	2196	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2120311	.	stem_loop	2163	2182	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2120311	.	polyA_site	2183	2196	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2120311	.	ribosome_entry_site	831	842	.	+	0	ID=BBa_K1045010;Name=BBa_K1045010
BBa_K2120311	.	non_covalent_binding_site	831	842	.	+	0	ID=annotation2360435;Name=inverse RBS;Parent=BBa_K1045010
BBa_K2120311	.	promoter	138	822	.	+	0	ID=BBa_K2120100;Name=BBa_K2120100
BBa_K2120311	.	promoter	138	1346	.	+	0	ID=annotation2525146;Name=promoter;Parent=BBa_K2120100
>BBa_K2120311
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagcggacccactttcacatttaagttgtttttctaatcc
gcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaat
aatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaac
ctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaa
ttgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagt
aaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtat
ggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctg
ctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctg
ttaatcactttacttttatctaatctggacattactagagtttctcctcttttactagaggttagcccaaaaaacgggt
atggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcata
gcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacgcgtttttgtc
atggctttggtcccgctttgttacagaatgcttttaataagcggggttaccggtttggttagcgagaagagccagtaaa
agacgcagtgacggcaatgtctgatgcaatatggacaattggtttcttctctgaatggcgggagtatgaaaagtatggc
tgaagcgcaaaatgatcccctgctgccgggatactcgtttaatgcccatctggtggcgggtttaacgccgattgaggcc
aacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagg
gggtggtgaaaaatcagggacgagaatttgtttgccgaccgggtgatattttgctgttcccgccaggagagattcatca
ctacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatgg
cttaactggccgtcaatatttgccaatacggggttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgt
ttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgtt
actgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatc
agcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcac
atcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgtatcagccaggcgaagctgctttt
gagcaccacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgggtattt
aaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgt
cataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatc
agtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggctt
cctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaat
cgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccg
ttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggact
acctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttac
ccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccg
gttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa
tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggt
tcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacag
tacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata