LOCUS       BBa_K2120322             884 bp    DNA     linear       26-Dec-2024
DEFINITION  J23116+B0032+tetR+B0015
ACCESSION   BBa_K2120322
VERSION     BBa_K2120322.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             66..682
                     /label=tetR
     misc_feature    683..716
                     /label=SsrA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             63..747
                     /label=BBa_C0040
     stem_loop       767..810
                     /label=stem_loop
     misc_feature    756..835
                     /label=BBa_B0010
     terminator      756..835
                     /label=BBa_B0010
     stem_loop       851..870
                     /label=T7 TE
     misc_feature    877..877
                     /label=stop
     polyA_site      871..884
                     /label=polya
     misc_feature    844..884
                     /label=BBa_B0012
     terminator      844..884
                     /label=BBa_B0012
     terminator      756..884
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagtcacaca ggaaagtact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      721 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc
      781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//