LOCUS       BBa_K2120414            1532 bp    DNA     linear       22-Dec-2024
DEFINITION  B0015+cI+B0034+J23109+pR+B0032+mazF+B0015
ACCESSION   BBa_K2120414
VERSION     BBa_K2120414.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..912
                     /label=BBa_K2120105
     misc_feature    921..932
                     /label=inverse RBS
     RBS             921..932
                     /label=BBa_K1045010
     promoter        1021..1026
                     /label=-10
     promoter        998..1003
                     /label=-35
     misc_feature    1008..1024
                     /label=OR1
     misc_feature    984..1000
                     /label=OR2
     misc_feature    984..1032
                     /label=BBa_R0051
     promoter        984..1032
                     /label=BBa_R0051
     RBS             1041..1053
                     /label=RBS-3\Weak
     RBS             1047..1050
                     /label=RBS
     misc_feature    1041..1053
                     /label=BBa_B0032
     RBS             1041..1053
                     /label=BBa_B0032
     CDS             1060..1395
                     /label=BBa_K2120008
     stem_loop       1415..1458
                     /label=stem_loop
     misc_feature    1404..1483
                     /label=BBa_B0010
     terminator      1404..1483
                     /label=BBa_B0010
     stem_loop       1499..1518
                     /label=T7 TE
     misc_feature    1525..1525
                     /label=stop
     polyA_site      1519..1532
                     /label=polya
     misc_feature    1492..1532
                     /label=BBa_B0012
     terminator      1492..1532
                     /label=BBa_B0012
     terminator      1404..1532
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt
      241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg
      301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag
      361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat
      421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc
      481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc
      541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg
      601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat
      661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa
      721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg
      781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt
      841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt
      901 ttttgtgctc attactagag tttctcctct tttactagag gctagcacag tccctaggac
      961 tgagctagct gtaaatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt
     1021 gataatggtt gctactagag tcacacagga aagtactaga tggtaagccg atacgtaccc
     1081 gatatgggcg atctgatttg ggttgatttt gacccgacaa aaggtagcga gcaagctgga
     1141 catcgtccag ctgttgtcct gagtcctttc atgtacaaca acaaaacagg tatgtgtctg
     1201 tgtgttcctt gtacaacgca atcaaaagga tatccgttcg aagttgtttt atccggtcag
     1261 gaacgtgatg gcgtagcgtt agctgatcag gtaaaaagta tcgcctggcg ggcaagagga
     1321 gcaacgaaga aaggaacagt tgccccagag gaattacaac tcattaaagc caaaattaac
     1381 gtactgattg ggtagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1441 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1501 ctcaccttcg ggtgggcctt tctgcgttta ta
//