LOCUS BBa_K2120414 1532 bp DNA linear 31-Oct-2024 DEFINITION B0015+cI+B0034+J23109+pR+B0032+mazF+B0015 ACCESSION BBa_K2120414 VERSION BBa_K2120414.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 CDS 138..912 /label=BBa_K2120105 misc_feature 921..932 /label=inverse RBS RBS 921..932 /label=BBa_K1045010 promoter 1021..1026 /label=-10 promoter 998..1003 /label=-35 misc_feature 1008..1024 /label=OR1 misc_feature 984..1000 /label=OR2 misc_feature 984..1032 /label=BBa_R0051 promoter 984..1032 /label=BBa_R0051 RBS 1041..1053 /label=RBS-3\Weak RBS 1047..1050 /label=RBS misc_feature 1041..1053 /label=BBa_B0032 RBS 1041..1053 /label=BBa_B0032 CDS 1060..1395 /label=BBa_K2120008 stem_loop 1415..1458 /label=stem_loop misc_feature 1404..1483 /label=BBa_B0010 terminator 1404..1483 /label=BBa_B0010 stem_loop 1499..1518 /label=T7 TE misc_feature 1525..1525 /label=stop polyA_site 1519..1532 /label=polya misc_feature 1492..1532 /label=BBa_B0012 terminator 1492..1532 /label=BBa_B0012 terminator 1404..1532 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt 241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg 301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag 361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat 421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc 481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc 541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg 601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat 661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa 721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg 781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt 841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt 901 ttttgtgctc attactagag tttctcctct tttactagag gctagcacag tccctaggac 961 tgagctagct gtaaatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt 1021 gataatggtt gctactagag tcacacagga aagtactaga tggtaagccg atacgtaccc 1081 gatatgggcg atctgatttg ggttgatttt gacccgacaa aaggtagcga gcaagctgga 1141 catcgtccag ctgttgtcct gagtcctttc atgtacaaca acaaaacagg tatgtgtctg 1201 tgtgttcctt gtacaacgca atcaaaagga tatccgttcg aagttgtttt atccggtcag 1261 gaacgtgatg gcgtagcgtt agctgatcag gtaaaaagta tcgcctggcg ggcaagagga 1321 gcaacgaaga aaggaacagt tgccccagag gaattacaac tcattaaagc caaaattaac 1381 gtactgattg ggtagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 1441 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 1501 ctcaccttcg ggtgggcctt tctgcgttta ta //