LOCUS       BBa_K2120416            1816 bp    DNA     linear       10-Jun-2024
DEFINITION  B0015+tetR+B0034+J23119+pTet+B0034+mRFP+B0015
ACCESSION   BBa_K2120416
VERSION     BBa_K2120416.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    831..842
                     /label=inverse RBS
     RBS             831..842
                     /label=BBa_K1045010
     promoter        851..885
                     /label=promoter
     promoter        851..885
                     /label=BBa_K2120101
     misc_feature    894..912
                     /label=TetR 1
     misc_feature    894..947
                     /label=BBa_R0040
     promoter        913..918
                     /label=-35
     misc_feature    919..937
                     /label=TetR 2
     promoter        936..941
                     /label=-10
     promoter        894..947
                     /label=BBa_R0040
     misc_feature    960..963
                     /label=conserved
     RBS             956..967
                     /label=BBa_B0034
     CDS             974..1648
                     /label=mrfp1
     misc_feature    1655..1679
                     /label=Help:Barcodes
     CDS             974..1679
                     /label=BBa_E1010
     stem_loop       1699..1742
                     /label=stem_loop
     misc_feature    1688..1767
                     /label=BBa_B0010
     terminator      1688..1767
                     /label=BBa_B0010
     stem_loop       1783..1802
                     /label=T7 TE
     misc_feature    1809..1809
                     /label=stop
     polyA_site      1803..1816
                     /label=polya
     misc_feature    1776..1816
                     /label=BBa_B0012
     terminator      1776..1816
                     /label=BBa_B0012
     terminator      1688..1816
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggtg atctacacta gcactatcag tgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cggacccact ttcacattta agttgttttt ctaatccgca
      241 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      301 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      361 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
      421 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
      481 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
      541 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
      601 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
      661 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
      721 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
      781 taatgcgctg ttaatcactt tacttttatc taatctggac attactagag tttctcctct
      841 tttactagag gctagcatta tacctaggac tgagctagct gtcaatacta gagtccctat
      901 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga
      961 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
     1021 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
     1081 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
     1141 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     1201 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
     1261 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
     1321 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     1381 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     1441 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     1501 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     1561 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     1621 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
     1681 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1741 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1801 cctttctgcg tttata
//